Rabbit Anti-TTF1 antibody | Sunlong Medical

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thyroid transcription factor1; NK2 homeobox 1; benign chorea; NK-2 homolog A; thyroid nuclear factor; thyroid transcription factor 1; NKX2-1; NKX2.1; BCH; BHC; NK-2; TEBP; TTF1; NKX2A; TITF1; TTF-1; NKX2.1; ZO1_HUMAN.

Cat:

SL10539R

Species Reactivity：

Human,Mouse,Rat,(predicted: Chicken,Cow,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human TTF1:121-220/371

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000Flow-Cyt=1μg/Testnot yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Lane 1: Mouse Thyroid gland tissue lysates Primary: Anti-TTF1 (SL10539R) at 1/200 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 38 kDaObserved band size: 42 kDaCell: A549Concentration:1:100Host/Isotype:Rabbit/IgG (blue)Flow cytometric analysis of Rabbit IgG isotype control (Cat#: SL10539R) on A549(green) compared with control in the absence of primary antibody (blue) followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG(H+L) secondary antibody .Blank control(blue): RSC96 Cells(fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice). Primary Antibody: Rabbit Anti-TTF1/AF647 Conjugated antibody (SL10539R-AF647), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/AF647(orange) ,used under the same conditions.

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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This gene encodes a protein initially identified as a thyroid-specific transcription factor. The encoded protein binds to the thyroglobulin promoter and regulates the expression of thyroid-specific genes but has also been shown to regulate the expression of genes involved in morphogenesis. Mutations and deletions in this gene are associated with benign hereditary chorea, choreoathetosis, congenital hypothyroidism, and neonatal respiratory distress, and may be associated with thyroid cancer. Multiple transcript variants encoding different isoforms have been found for this gene. This gene shares the symbol/alias 'TFF1' with another gene, transcription termination factor 1, which plays a role in ribosomal gene transcription. [provided by RefSeq, Apr 2011]

Function:
Transcription factor that binds and activates the promoter of thyroid specific genes such as thyroglobulin, thyroperoxidase, and thyrotropin receptor. Crucial in the maintenance of the thyroid differentiation phenotype. May play a role in lung development and surfactant homeostasis.

Subcellular Location:
Nucleus.

Tissue Specificity:
Thyroid, lung and CNS. Expressed in restricted regions of the developing brain within the diencephalon, in parts of the hypothalamus and neurohypophysis, and in the telencephalon.

Post-translational modifications:
Phosphorylated on serine residues by STK3.

Similarity:
Belongs to the NK-2 homeobox family.
Contains 1 homeobox DNA-binding domain.

SWISS:
P43699

Gene ID:
7080

Database links:

Entrez Gene: 7080 Human

Entrez Gene: 21869 Mouse

Entrez Gene: 25628 Rat

Omim: 600635 Human

SwissProt: P43699 Human

SwissProt: P50220 Mouse

SwissProt: P23441 Rat

Unigene: 94367 Human

Unigene: 89972 Mouse

Unigene: 34265 Rat

Picture

Cell: A549
Concentration:1:100
Host/Isotype:Rabbit/IgG (blue)
Flow cytometric analysis of Rabbit IgG isotype control (Cat#: SL10539R) on A549(green) compared with control in the absence of primary antibody (blue) followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG(H+L) secondary antibody .

Blank control(blue): RSC96 Cells(fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice).
Primary Antibody: Rabbit Anti-TTF1/AF647 Conjugated antibody (SL10539R-AF647), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG/AF647(orange) ,used under the same conditions.

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