Insulin like growth factor binding protein 4 (IGFBP4) is produced from a DNA sequence encoding the human IGFBP4 protein. Mature human IGFBP4 has a calculated molecular mass of approximately 26 kDa. Due to glycosylation, the recombinant protein migrates as a 32 kDa and 25 kDa protein under reducing and non reducing conditions, respectively. Human IGFBP4 has a potential N-linked glycosylation site and shares approximately 90% amino acid sequence identity with both mouse and rat IGFBP4. IGFBP4 is a member of the superfamily of insulin-like growth factor (IGF) binding proteins which include six high affinity IGF binding proteins (IGFBP) and at least four low affinity binding proteins referred to as IGFBP related proteins (IGFBPrP). IGFBP4 functions as an inhibitor of IGF action and its main function may be to protect cells from overstimulation by IGFs or to allow activation of alternate transmembrane signaling pathways that are inhibited by IGF exposure. IGFBP4 is expressed in multiple tissues including adrenal, testis, spleen, heart, lung, kidney, liver, stomach, hypothalamus, and brain cortex.
Function:
IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
Subunit:
Binds IGF2 more than IGF1.
Subcellular Location:
Secreted.
Similarity:
Contains 1 IGFBP N-terminal domain.
Contains 1 thyroglobulin type-1 domain.
SWISS:
P22692
Gene ID:
3487
Database links:
Entrez Gene: 374271 Chicken
Entrez Gene: 282262 Cow
Entrez Gene: 3487 Human
Entrez Gene: 16010 Mouse
Entrez Gene: 72622 Rat
Entrez Gene: 443470 Sheep
Omim: 146733 Human
SwissProt: Q05716 Cow
SwissProt: P22692 Human
SwissProt: P47879 Mouse
SwissProt: P24854 Pig
SwissProt: P21744 Rat
SwissProt: Q28893 Sheep
Unigene: 462998 Human
Unigene: 233799 Mouse
Unigene: 160666 Rat
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Sample:
Liver (Mouse) Lysate at 40 ug
Primary: Anti-IGFBP4 (SL10585R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 26 kD
Observed band size: 27 kD
Tissue/cell: Rat adrenal tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-IGFBP4 Polyclonal Antibody, Unconjugated(SL10585R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
Tissue/cell: Rat spleen tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-IGFBP4 Polyclonal Antibody, Unconjugated(SL10585R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
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