Rabbit Anti-phospho-JNK1 (Thr183) antibody

Home > Product > Antibody > Rabbit Anti-phospho-JNK1 (Thr183) antibody

JNK1 (phospho T183); p-JNK1 (phospho T183); AI849689; c Jun N terminal kinase 1; C-JUN kinase 1; c-Jun N-terminal kinase 1; EC 2.7.11.24; JNK 1; JNK; JNK-46; JNK1A2; JNK21B1/2; MAP kinase 8; MAPK 8; mapk8; Mitogen activated protein kinase 8;

Cat:

SL17591R

Species Reactivity：

Human,Rat,(predicted: Mouse,Chicken,Dog,Pig,Cow,Horse,Zebrafish,Sheep,)

Immunogen：

KLH conjugated synthesised phosphopeptide derived from human JNK1 around the phosphorylation site of Thr183:MM(p-T)PY

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Hela-UV(Human) Cell Lysate at 30 ug293T-UV(Human) Cell Lysate at 30 ugJurkat-UV(Human) Cell Lysate at 30 ugPrimary: Anti-phospho-JNK1 (Thr183) (SL17591R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 50 kDObserved band size: 50 kDSample: U251(Human) Cell Lysate at 30 ugPrimary: Anti-phospho-JNK1 (Thr183) (SL17591R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 50 kDObserved band size: 50 kDParaformaldehyde-fixed, paraffin embedded (rat heart tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (p-JNK1 (Thr183)) Polyclonal Antibody, Unconjugated (SL17591R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

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Unit:

Price: $256.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Five alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jun 2013]

Function:
Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells (By similarity). Phosphorylates heat shock factor protein 4 (HSF4). JNK1 isoforms display different binding patterns: beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta-2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms.

Post-translational modifications:
Dually phosphorylated on Thr-183 and Tyr-185, which activates the enzyme.

Similarity:
Belongs to the protein kinase superfamily.
CMGC Ser/Thr protein kinase family.
MAP kinase subfamily.
Contains 1 protein kinase domain.

SWISS:
P45983

Gene ID:
5599

Database links:

Entrez Gene: 5599 Human

Entrez Gene: 26419 Mouse

Entrez Gene: 116554 Rat

Omim: 601158 Human

SwissProt: P45983 Human

SwissProt: Q91Y86 Mouse

SwissProt: P49185 Rat

Unigene: 138211 Human

Unigene: 21495 Mouse

Unigene: 4090 Rat

Picture

Sample:
U251(Human) Cell Lysate at 30 ug
Primary: Anti-phospho-JNK1 (Thr183) (SL17591R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 50 kD

Paraformaldehyde-fixed, paraffin embedded (rat heart tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (p-JNK1 (Thr183)) Polyclonal Antibody, Unconjugated (SL17591R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

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Specific References (9) | SL17591R has been referenced in 9 publications.

[IF=5.293] Liu Jiawei. et al. Lactobacillus rhamnosus GR-1 Alleviates Escherichia coli-Induced Inflammation via NF-κB and MAPKs Signaling in Bovine Endometrial Epithelial Cells. Front Cell Infect Mi. 2022 Mar;0:199 WB ; Cattle.

PubMed:35310848

[IF=3.352] Yusong Miao. et al. Methylsulfonylmethane ameliorates inflammation via NF-κB and ERK/JNK-MAPK signaling pathway in chicken trachea and HD11 cells during Mycoplasma gallisepticum infection. Poultry Sci. 2022 Jan;:101706 WB ; Chicken.

PubMed:35121233

[IF=2.027] Li QH et al. Effect of heat stress on mitogen-activated protein kinases in the hypothalamic− pituitary− gonadal axis of developing Wenchang chicks. Poultry Science.2019. IHSLCP&WB ; chick.

PubMed:doi:10.3382/ps/pez499

[IF=4.064] Ming Ni. et al. Sox11 Modified Tendon-Derived Stem Cells Promote the Repair of Osteonecrosis of Femoral Head:. Cell Transplant. 2021;(): WB ; Rabbit.

PubMed:34699265

[IF=2.094] Jing Li. et al. Melatonin ameliorates cypermethrin-induced impairments by regulating oxidative stress, DNA damage and apoptosis in porcine Sertoli cells. Theriogenology. 2021 Mar;: WB ; Pig.

PubMed:10.1016/j.theriogenology.2021.03.011

[IF=1.713] Jian Wang. et al. A respiratory commensal bacterium acts as a risk factor for Mycoplasma gallisepticum infection in chickens. Vet Immunol Immunop. 2020 Dec;230:110127 WB ; Chicken.

PubMed:33080531

[IF=1.713] Jian Wang. et al. Lactobacillus salivarius ameliorated Mycoplasma gallisepticum-induced inflammatory injury and secondary Escherichia coli infection in chickens: Involvement of intestinal microbiota. Vet Immunol Immunop. 2021 Mar;233:110192 WB ; Chicken.

PubMed:33476924

[IF=1.723] Wang X et al. Wilms' tumour 1 (WT1) negatively regulates the expression of connexin 43 via a non-canonical Wnt signalling pathway in cultured bovine Sertoli cells. Reprod Fertil Dev. 2020 Feb 6. WB ; Bovine.

PubMed:6423428

[IF=3.166] Chang L et al. Melamine causes testicular toxicity by destroying blood-testis barrier in piglets.Toxicol Lett. 2018 Oct 15;296:114-124. WB ; Piglet.

PubMed:3005548