SPRED2 is a member of the Sprouty (see SPRY1; MIM 602465)/SPRED family of proteins that regulate growth factor-induced activation of the MAP kinase cascade (see MAPK1; MIM 176948) (Nonami et al., 2004 [PubMed 15465815]).[supplied by OMIM, Mar 2008]
Function:
Tyrosine kinase substrate that inhibits growth-factor-mediated activation of MAP kinase.
Subunit:
Interacts with Ras (By similarity). Homodimer and heterodimer. Able to interact with SPRED1 to form heterodimers.
Subcellular Location:
Cell Membrane - peripheral membrane protein and Cytoplasmic.
Tissue Specificity:
Expressed in liver, skin, small intestine, salivary gland and prostate.
Post-translational modifications:
Phosphorylated on tyrosine. Phosphorylation of Tyr-228 and Tyr-231 are required for ubiquitination.
Ubiquitinated; leading to degradation by the proteasome.
Similarity:
Contains 1 KBD domain.
Contains 1 SPR (sprouty) domain.
Contains 1 WH1 domain.
SWISS:
Q7Z698
Gene ID:
200734
Database links:
Entrez Gene: 200734 Human
Entrez Gene: 114716 Mouse
Entrez Gene: 305539 Rat
Omim: 609292 Human
SwissProt: Q7Z698 Human
SwissProt: Q924S7 Mouse
SwissProt: Q3C2P8 Rat
Unigene: 59332 Human
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Paraformaldehyde-fixed, paraffin embedded (mouse pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SPRED2) Polyclonal Antibody, Unconjugated (SL17686R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SPRED2) Polyclonal Antibody, Unconjugated (SL17686R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SPRED2) Polyclonal Antibody, Unconjugated (SL17686R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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