Rabbit Anti-LRRC33 antibody

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ELLP3030; GARPL1; NRROS_HUMAN; Leucine rich repeat containing protein 33; Lrcc33; UNQ3030.

Cat:

SL18379R

Species Reactivity：

Human,Mouse,Rat,(predicted: Rabbit,)

Immunogen：

KLH conjugated synthetic peptide derived from human LRRC33:501-600/692<Extracellular>

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Bone(Rat) Cell Lysate at 40 ugThymus(Rat) Cell Lysate at 40 ugPrimary: Anti-LRRC33 (SL18379R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 74 kDObserved band size: 74 kDParaformaldehyde-fixed, paraffin embedded (Mouse skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (LRRC33) Polyclonal Antibody, Unconjugated (SL18379R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (SL0295G-cy3) for 90 minutes, and DAPI for nuclei staining.Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (LRRC33) Polyclonal Antibody, Unconjugated (SL18379R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (SL0295G-cy3) for 90 minutes, and DAPI for nuclei staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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LRRC33 is a 692 amino acid protein that contains 17 LRR repeats. The gene encoding LRRC33 maps to chromosome 3, which encodes over 1,100 genes. Particular regions of the chromosome 3 short arm are deleted in many types of cancer cells. Marfan syndrome, porphyria, von Hippel-Lindau syndrome, osteogenesis imperfecta and Charcot-Marie-Tooth disease are a few of the numerous genetic diseases associated with chromosome 3.

Function:
Negative regulator of reactive oxygen species (ROS) that limits ROS production by phagocytes during inflammatory response, thereby playing a role during host defense. Acts via direct interaction with CYBB/NOX2 monomer that impairs interaction between CYBB/NOX2 and CYBA/p22-phox and formation of a stable NOX2 complex.
May play a critical role in desensitizing TLR signaling through inhibition of Toll-like receptor-mediated NF-kappa-B activation and cytokine production.

Subunit:
Interacts with CYBB/NOX2; the interaction is direct By similarity. Interacts (via LRR repeats) with TLR2, TLR3, TLR4, TLR9 and probably other Toll-like receptors.

Subcellular Location:
Membrane; Single pass type I membrane protein.

Tissue Specificity:
Ubiquitous, with high level of expression found in bone marrow, thymus, liver, lung, intestine and spleen.

Similarity:
Contains 21 LRR (leucine-rich) repeats.

SWISS:
Q86YC3

Gene ID:
375387

Database links:

Entrez Gene: 375387 Human

Entrez Gene: 224109 Mouse

Entrez Gene: 303875 Rat

SwissProt: Q86YC3 Human

SwissProt: Q8BMT4 Mouse

SwissProt: Q5BK65 Rat

Picture

Paraformaldehyde-fixed, paraffin embedded (Mouse skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (LRRC33) Polyclonal Antibody, Unconjugated (SL18379R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (SL0295G-cy3) for 90 minutes, and DAPI for nuclei staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (LRRC33) Polyclonal Antibody, Unconjugated (SL18379R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (SL0295G-cy3) for 90 minutes, and DAPI for nuclei staining.

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