Rabbit Anti-ZBTB9/ZNF919 antibody

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3930402F13Rik; DASS-97D12.7; MGC23166; ZBTB9; ZBTB9_HUMAN; Zinc finger and BTB domain containing 9; Zinc finger and BTB domain containing protein 9; Zinc finger and BTB domain-containing protein 9; ZNF919.

Cat:

SL18457R

Species Reactivity：

Human,Mouse,Rat,(predicted: Pig,Cow,Horse,Rabbit,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human ZBTB9/ZNF919:331-430/473

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Du145(Human) Cell Lysate at 40 ugLovo(Human) Cell Lysate at 40 ugPrimary: Anti-ZBTB9/ZNF919 (SL18457R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 51 kDObserved band size: 51 kDParaformaldehyde-fixed, paraffin embedded (Rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ZBTB9/ZNF919) Polyclonal Antibody, Unconjugated (SL18457R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ZBTB9/ZNF919) Polyclonal Antibody, Unconjugated (SL18457R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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ZBTB9 is a 473 amino acid member of the Krüppel C2H2-type zinc-finger protein family. Localized to the nucleus, ZBTB9 contains a BTB domain, also known as a POZ domain, which inhibits DNA binding and mediates homotypic and heterotypic dimerization. Characteristics of the BTB domain suggest that ZBTB43 functions as a transcription regulator.

Function:
May be involved in transcriptional regulation.

Subcellular Location:
Nucleus.

Similarity:
Contains 1 BTB (POZ) domain.
Contains 2 C2H2-type zinc fingers.

SWISS:
Q96C00

Gene ID:
221504

Database links:

Entrez Gene: 221504 Human

Entrez Gene: 474156 Mouse

SwissProt: Q96C00 Human

SwissProt: Q8CDC7 Mouse

Unigene: 59365 Human

Unigene: 328890 Mouse

Picture

Paraformaldehyde-fixed, paraffin embedded (Rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ZBTB9/ZNF919) Polyclonal Antibody, Unconjugated (SL18457R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ZBTB9/ZNF919) Polyclonal Antibody, Unconjugated (SL18457R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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