Rabbit Anti-ZFP30 antibody | Sunlong Medical

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KIAA0961; ZFP 30; Zfp-30; ZFP30; ZFP30_HUMAN; Zinc finger protein 30 homolog; Zinc finger protein 745; ZNF745.

Cat:

SL19124R

Species Reactivity：

Human,

Immunogen：

KLH conjugated synthetic peptide derived from human ZFP30:101-200/519

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: MDA-MB-231(Human) Cell Lysate at 30 ugPrimary: Anti-ZFP30 (SL19124R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 62 kDObserved band size: 62 kDSample: 293T(Human) Cell Lysate at 30 ugU251(Human) Cell Lysate at 30 ugU87MG(Human) Cell Lysate at 30 ugMDA-MB-231(Human) Cell Lysate at 30 ugPrimary: Anti-ZFP30 (SL19124R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 62 kDObserved band size: 62 kD Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ZFP30) Polyclonal Antibody, Unconjugated (SL19124R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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ZFP30 is a 519 amino acid protein that localizes to the nucleus and is composed of thirteen C2H2-type zinc fingers and one KRAB domain. ZFP30 is encoded by a gene located on human chromosome 19, which consists of over 63 million bases, houses approximately 1,400 genes and is recognized for having the greatest gene density of the human chromosomes. It is the genetic home for a number of immunoglobulin (Ig) superfamily members, including the killer cell and leukocyte Ig-like receptors, a number of ICAMs, the CEACAM and PSG families, and Fc receptors (FcRs).

Function:
May be involved in transcriptional regulation.

Subcellular Location:
Nucleus.

Similarity:
Belongs to the krueppel C2H2-type zinc-finger protein family.
Contains 13 C2H2-type zinc fingers.
Contains 1 KRAB domain.

SWISS:
Q9Y2G7

Gene ID:
22835

Database links:

Entrez Gene: 22835 Human

Entrez Gene: 22693 Mouse

SwissProt: Q9Y2G7 Human

SwissProt: Q60585 Mouse

Unigene: 716719 Human

Picture

Sample:
293T(Human) Cell Lysate at 30 ug
U251(Human) Cell Lysate at 30 ug
U87MG(Human) Cell Lysate at 30 ug
MDA-MB-231(Human) Cell Lysate at 30 ug
Primary: Anti-ZFP30 (SL19124R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 62 kD
Observed band size: 62 kD

Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ZFP30) Polyclonal Antibody, Unconjugated (SL19124R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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