Rabbit Anti-ZFYVE26 antibody

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DKFZp686F19106; DKFZp781H1112; FYVE domain-containing centrosomal protein; FYVE-CENT; KIAA0321; Spastic paraplegia 15 (complicated autosomal recessive); Spastizin; SPG15; ZFY26_HUMAN; ZFYVE 26; ZFYVE26; Zinc finger FYVE domain containing 26; Z

Cat:

SL19159R

Species Reactivity：

Human,Mouse,(predicted: Rat,Dog,Pig,Cow,Horse,Rabbit,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human ZFYVE26:2151-2250/2539

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Brain (Mouse) Lysate at 40 ugU251 (Human) CellLysate at 30 ugU87MG (Human) CellLysate at 30 ugPrimary: Anti-ZFYVE26 (SL19159R) at 1/500 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 285 kDObserved band size: 285 kDSample: Brain (Mouse) Lysate at 40 ugPrimary: Anti-ZFYVE26 (SL19159R) at 1/500 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 285 kDObserved band size: 285 kDParaformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ZFYVE26) Polyclonal Antibody, Unconjugated (SL19159R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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This gene encodes a protein which contains a FYVE zinc finger binding domain. The presence of this domain is thought to target these proteins to membrane lipids through interaction with phospholipids in the membrane. Mutations in this gene are associated with autosomal recessive spastic paraplegia-15. [provided by RefSeq, Oct 2008]

Function:
Phosphatidylinositol 3-phosphate-binding protein required for the abcission step in cytokinesis: recruited to the midbody during cytokinesis and acts as a regulator of abcission. May also be required for efficient homologous recombination DNA double-strand break repair.

Subcellular Location:
Cytoplasm > cytoskeleton > microtubule organizing center > centrosome. Midbody. Localizes to the centrosome during all stages of the cell cycle. Recruited to the midbody during cytokinesis by KIF13A.

Tissue Specificity:
Strongest expression in the adrenal gland, bone marrow, adult brain, fetal brain, lung, placenta, prostate, skeletal muscle, testis, thymus, and retina. Intermediate levels are detected in other structures, including the spinal cord.

DISEASE:
Spastic paraplegia 15, autosomal recessive

Similarity:
Contains 1 FYVE-type zinc finger.

SWISS:
Q68DK2

Gene ID:
23503

Database links:

Entrez Gene: 51882 Cow

Entrez Gene: 23503 Human

Entrez Gene: 211978 Mouse

Entrez Gene: 314265 Rat

Omim: 612012 Human

SwissProt: E1BLZ4 Cow

SwissProt: Q68DK2 Human

SwissProt: Q5DU37 Mouse

SwissProt: D4A8G9 Rat

Unigene: 19641 Human

Unigene: 297919 Mouse

Unigene: 16048 Rat

Picture

Sample:
Brain (Mouse) Lysate at 40 ug
Primary: Anti-ZFYVE26 (SL19159R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 285 kD
Observed band size: 285 kD

Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ZFYVE26) Polyclonal Antibody, Unconjugated (SL19159R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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