Rabbit Anti-MYO16 antibody | Sunlong Medical

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Kiaa0865; Myo16; MYO16 variant protein; MYO16_HUMAN; Myo16b; Myosin heavy chain Myr 8; Myosin-XVI; MYR8; Neuronal tyrosine-phosphorylated phosphoinositide-3-kinase; neuronal tyrosine-phosphorylated phosphoinositide-3-kinase adapter 3; NYAP3; RP

Cat:

SL19164R

Species Reactivity：

Mouse,Rat,Fruit Fly,(predicted: Human,Chicken,Zebrafish,)

Immunogen：

KLH conjugated synthetic peptide derived from human MYO16:1251-1350/1858

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Cerebrum (Mouse) Lysate at 40 ugCerebrum (Rat) Lysate at 40 ugPrimary: Anti-MYO16 (SL19164R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 206 kDObserved band size: 123 kDSample: Brain (Mouse) Lysate at 40 ugBrain (Rat) Lysate at 40 ugPrimary: Anti-MYO16 (SL19164R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 206 kDObserved band size: 123 kD Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MYO16) Polyclonal Antibody, Unconjugated (SL19164R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Myosin XVI is a 1,858 amino acid protein that localizes to the cytoplasm and contains one IQ domain, two myosin head-like domains and seven ANK repeats. Existing as an unconventional Myosin, Myosin XVI is involved in intracellular movements related to Actin filaments and is thought to interact specifically with PP1 alpha and PP1 gamma, possibly playing a role in PP1-associated brain development. Multiple isoforms of Myosin XVI exist due to alternative splicing events.

Subcellular Location:
Cytoplasm. Found in puncta in soma and processes of astrocytes and dissociated cerebellar cells with the morphology of migrating granule cells.

Similarity:
Contains 7 ANK repeats.
Contains 1 IQ domain.
Contains 2 myosin head-like domains.

SWISS:
Q9Y6X6

Gene ID:
23026

Database links:

Entrez Gene: 23026 Human

Entrez Gene: 244281 Mouse

Entrez Gene: 192253 Rat

SwissProt: Q9Y6X6 Human

SwissProt: Q5DU14 Mouse

SwissProt: Q9ERC1 Rat

Unigene: 656587 Human

Unigene: 422761 Mouse

Unigene: 207204 Rat

Picture

Sample:
Brain (Mouse) Lysate at 40 ug
Brain (Rat) Lysate at 40 ug
Primary: Anti-MYO16 (SL19164R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 206 kD
Observed band size: 123 kD

Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MYO16) Polyclonal Antibody, Unconjugated (SL19164R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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