Rabbit Anti-ABI3 antibody | Sunlong Medical

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ABI gene family member 3; Nesh; New molecule including SH3; ABI3_HUMAN.

Cat:

SL10798R

Species Reactivity：

Human,Mouse,(predicted: Rat,Dog,Pig,Cow,Rabbit,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human ABI3:21-120/366

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Heart (Mouse) Lysate at 40 ugPrimary: Anti- ABI3 (SL10798R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 39 kDObserved band size: 50 kDSample: HUVEC(Human) Cell Lysate at 30 ugPrimary: Anti-ABI3 (SL10798R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 39 kDObserved band size: 48 kDTissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-ABI3 Polyclonal Antibody, Unconjugated(SL10798R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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ABI3 is thought to inhibit tumor metastasis. In vitro, ABI3 reduces cell motility.

Function:
May inhibit tumor metastasis. In vitro, reduces cell motility.

Subunit:
May interact with PAK1 and PAK2. Probably interacts with TARSH.

Subcellular Location:
Cytoplasm. Note=Colocalizes with PAK2 at leading edge of cells.

Tissue Specificity:
Expressed in heart, lung, liver, pancreas, kidney, placenta and at low levels in brain and skeletal muscle.

Similarity:
Belongs to the ABI family.
Contains 1 SH3 domain.

SWISS:
Q9P2A4

Gene ID:
51225

Database links:

Entrez Gene: 51225 Human

Entrez Gene: 66610 Mouse

Entrez Gene: 303476 Rat

Omim: 606363 Human

SwissProt: Q9P2A4 Human

SwissProt: Q8BYZ1 Mouse

Unigene: 130719 Human

Picture

Sample:
HUVEC(Human) Cell Lysate at 30 ug
Primary: Anti-ABI3 (SL10798R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 39 kD
Observed band size: 48 kD

Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-ABI3 Polyclonal Antibody, Unconjugated(SL10798R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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