The protein encoded by this gene is an integral membrane protein involved in gamma-aminobutyric acid (GABA) and glycine uptake into synaptic vesicles. The encoded protein is a member of amino acid/polyamine transporter family II. [provided by RefSeq, Jul 2008]
Function:
Involved in the uptake of GABA and glycine into the synaptic vesicles.
Subcellular Location:
Cytoplasmic vesicle membrane.
Tissue Specificity:
Retina. Expressed throughout the horizontal cells or more specifically at the terminals.
Similarity:
Belongs to the amino acid/polyamine transporter 2 family.
SWISS:
Q9H598
Gene ID:
140679
Database links:
Entrez Gene: 140679 Human
Entrez Gene: 22348 Mouse
Entrez Gene: 83612 Rat
SwissProt: Q9H598 Human
SwissProt: O35633 Mouse
SwissProt: O35458 Rat
Unigene: 179080 Human
Unigene: 143404 Mouse
Unigene: 413854 Mouse
Unigene: 10846 Rat
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Sample:heart (Mouse) Lysate at 40 ug
Primary: Anti-SLC32A1(SL19821R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 57kD
Observed band size: 60kD
Sample:Cerebellum (Mouse) Lysate at 40 ug
Primary: Anti-SLC32A1(SL19821R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 57kD
Observed band size: 57kD
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-SLC32A1 Polyclonal Antibody, Unconjugated(SL19821R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
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