Rabbit Anti-Rho guanine exchange factor 16 antibody

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ARHGEF 16; ARHGEF16; ARHGG_HUMAN; Ephexin 4; Ephexin-4; EPHEXIN4; GEF16; NBR; Putative Neuroblastoma Protein; Rho guanine exchange factor (GEF) 16; Rho guanine nucleotide exchange factor (GEF) 16; Rho guanine nucleotide exchange factor 16.

Cat:

SL19870R

Species Reactivity：

Mouse,(predicted: Human,Horse,)

Immunogen：

KLH conjugated synthetic peptide derived from human RHEBL1:151-250/709

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample:Thymus (Mouse) Lysate at 40 ugPrimary: Anti-Rho guanine exchange factor 16(SL19870R)at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 80kDObserved band size: 80kDSample:Placenta (Mouse) Lysate at 40 ugPrimary: Anti-Rho guanine exchange factor 16(SL19870R)at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 80kDObserved band size: 80kDParaformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Rho guanine exchange factor 16) Polyclonal Antibody, Unconjugated (SL19870R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Although the specific function of this protein is not known yet, it is thought to be involved in protein-protein and protein-lipid interactions. [provided by RefSeq, Jul 2008]

Function:
Guanyl-nucleotide exchange factor of the RHOG GTPase stimulating the exchange of RHOG-associated GDP for GTP. May play a role in chemotactic cell migration by mediating the activation of RAC1 by EPHA2. May also activate CDC42 and mediate activation of CDC42 by the viral protein HPV16 E6.

Subunit:
Interacts with ELMO2, EPHA2, RAC1 and RHOG; mediates activation of RAC1 by EPHA2. Interacts with TAX1BP3 (via PDZ domain). May interact with CDC42; stimulated by HPV16 E6.

Subcellular Location:
Cytoplasm.

Similarity:
Contains 1 DH (DBL-homology) domain.
Contains 1 PH domain.
Contains 1 SH3 domain.

SWISS:
Q5VV41

Gene ID:
27237

Database links:

Entrez Gene: 27237 Human

Entrez Gene: 230972 Mouse

SwissProt: Q5VV41 Human

SwissProt: Q3U5C8 Mouse

Unigene: 87435 Human

Unigene: 38481 Mouse

Picture

Sample:Placenta (Mouse) Lysate at 40 ug
Primary: Anti-Rho guanine exchange factor 16(SL19870R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 80kD
Observed band size: 80kD

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Rho guanine exchange factor 16) Polyclonal Antibody, Unconjugated (SL19870R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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