Sample: Lane 1: Cerebrum (Mouse) Lysate at 40 ug Lane 2: Lung (Mouse) Lysate at 40 ug Lane 3: SH-SY5Y (Human) Cell Lysate at 30 ug Lane 4: 293T (Human) Cell Lysate at 30 ug Lane 5: MCF-7 (Human) Cell Lysate at 30 ug Lane 6: Jurkat (Human) Cell Lysate at 30 ug Primary: Anti-phospho-Parkin (Ser65) (SL19882R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52 kD Observed band size: 50 kD
Sample: Thymus (Mouse) Lysate at 40 ug Liver (Mouse) Lysate at 40 ug Lung (Mouse) Lysate at 40 ug Skeletal muscle (Mouse) Lysate at 40 ug Primary: Anti- phospho-Parkin (Ser65) (SL19882R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52 kD Observed band size: 52 kD
Sample: A673(Human)Cell Lysate at 40 ug
Primary: Anti-p-Parkin(Ser65) (SL10196R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52 kD
Observed band size: 52 kD
Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Parkin (Ser65)) Polyclonal Antibody, Unconjugated (SL19882R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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