This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex. [provided by RefSeq, Jul 2008]
Function:
Essential component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Also required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA).
Subcellular Location:
Cytoplasm. Membrane.
Tissue Specificity:
Ubiquitous. Highly expressed in heart, brain, placenta, skeletal muscle, spleen, thymus, testis, ovary, small intestine, kidney and colon.
Similarity:
Belongs to the VPS35 family.
SWISS:
Q96QK1
Gene ID:
55737
Database links:
Entrez Gene: 521864 Cow
Entrez Gene: 100133770 Human
Entrez Gene: 55737 Human
Entrez Gene: 65114 Mouse
Entrez Gene: 25479 Rat
Omim: 601501 Human
SwissProt: Q2HJG5 Cow
SwissProt: Q96QK1 Human
SwissProt: Q9EQH3 Mouse
Unigene: 454528 Human
Unigene: 296520 Mouse
Unigene: 391632 Mouse
Unigene: 161720 Rat
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Sample:
Thymus (Mouse) Lysate at 40 ug
Primary: Anti-VPS35 (SL12772R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 91 kD
Observed band size: 91 kD
Sample:
MOLT-4(Human) Cell Lysate at 30 ug
Primary: Anti-VPS35 (SL12772R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 91 kD
Observed band size: 91 kD
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (VPS35) Polyclonal Antibody, Unconjugated (SL12772R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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