Rabbit Anti-SFRS15 antibody

Home > Product > Antibody > Rabbit Anti-SFRS15 antibody

CTD-binding SR-like protein RA4; DKFZp434E098; FLJ23364; KIAA1172; Pre mRNA splicing SR protein rA4; SCAF4; SFR15_HUMAN; Splicing factor arginine/serine rich 15; Splicing factor serine alanine 15; SR like CTD associated factor 4; SR-related and

Cat:

SL21109R

Species Reactivity：

Human,Mouse,(predicted: Rat,Dog,Pig,Cow,Horse,Rabbit,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human SFRS15:501-600/1147

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Thymus (Mouse) Lysate at 40 ugK562(Human) Cell Lysate at 30 ugHL60(Human) Cell Lysate at 30 ugSIHA(Human) Cell Lysate at 30 ugPrimary: Anti- SFRS15 (SL21109R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 126 kDObserved band size: 126 kDSample: Testis (Mouse) Lysate at 40 ugPrimary: Anti-SFRS15 (SL21109R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 126 kDObserved band size: 126 kDParaformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SFRS15) Polyclonal Antibody, Unconjugated (SL21109R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

prev next

Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
Feedback Wall

This gene likely encodes a member of the arginine/serine-rich splicing factor family. A similar protein in Rat appears to bind the large subunit of RNA polymerase II and provide a link between transcription and pre-mRNA splicing. Alternatively spliced transcript variants have been described. [provided by RefSeq, Feb 2009]

Function:
May act to physically and functionally link transcription and pre-mRNA processing.

Subcellular Location:
Nucleus.

Similarity:
Belongs to the splicing factor SR family.
Contains 1 CID domain.
Contains 1 RRM (RNA recognition motif) domain.

SWISS:
O95104

Gene ID:
57466

Database links:

Entrez Gene: 57466 Human

Entrez Gene: 224432 Mouse

Entrez Gene: 245924 Rat

SwissProt: O95104 Human

SwissProt: Q63627 Rat

Unigene: 17255 Human

Unigene: 439811 Mouse

Picture

Sample:
Testis (Mouse) Lysate at 40 ug
Primary: Anti-SFRS15 (SL21109R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 126 kD
Observed band size: 126 kD

Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SFRS15) Polyclonal Antibody, Unconjugated (SL21109R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Product Feedback Wall