OCT embedded (Rat spleen), acetone-fixed; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD68) Polyclonal Antibody, Unconjugated (SL20403R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (SL0295G-FITC) for 90 minutes, and DAPI for nuclei staining.
Blank control: RSC96 cells (blue).
Primary Antibody: Rabbit Anti-CD68 antibody(SL20403R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG (orange) ,used under the same conditions.
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.