Rabbit Anti-Livin antibody | Sunlong Medical

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Baculoviral IAP repeat containing 7; Baculoviral IAP repeat containing protein 7; BIRC 7; BIRC7; BIRC7_HUMAN; KIAP; Kidney inhibitor of apoptosis protein; LIVIN; Livin inhibitor of apotosis; Melanoma inhibitor of apoptosis protein; ML IAP; MLIAP; RING fin

Cat:

SL20731R

Species Reactivity：

Human,Rat,(predicted: Mouse,Cow,Rabbit,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human Livin:41-100/298

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500Flow-Cyt=1μg/TestIF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Hela(Human) Cell Lysate at 30 ugPrimary: Anti- Livin (SL20731R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 33 kDObserved band size: 33 kD Paraformaldehyde-fixed, paraffin embedded (Rat skin); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Livin) Polyclonal Antibody, Unconjugated (SL20731R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Blank control (blue line): Hep G2 (fixed with 70% ethanol (Overmight at 4℃) and then permeabilized with 90% ice-cold methanol for 30 min at -20℃). Primary Antibody (green line): Rabbit Anti-Nanog antibody (SL20731R),Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE,Dilution: 1μg /test. Blank control: HepG2. Primary Antibody (green line): Rabbit Anti-Livin antibody (SL20731R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647Dilution: 1μg /test. ProtocolThe cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Livin is a novel member of the IAP protein family and contains a single baculoviral IAP repeat (BIR) domain and a RING finger domain and has two isoforms termed Livin alpha and Livin beta. The BIR domain is essential for inhibitory activity and interacts with caspases, while the RING finger domain sometimes enhances antiapoptotic activity but does not inhibit apoptosis alone. The two isoforms of Livin appear to have different functions and tissue distributions.

Function:
Protects against apoptosis induced by TNF or by chemical agents such as adriamycin, etoposide or staurosporine. Suppression of apoptosis is mediated by activation of MAPK8/JNK1, and possibly also of MAPK9/JNK2. This activation depends on TAB1 and NR2C2/TAK1. In vitro, inhibits caspase-3 and proteolytic activation of pro-caspase-9. Isoform 1 blocks staurosporine-induced apoptosis. Isoform 2 blocks etoposide-induced apoptosis.

Subcellular Location:
Nucleus. Cytoplasm. Nuclear, and in a filamentous pattern throughout the cytoplasm.

Tissue Specificity:
Isoform 1 and isoform 2 are expressed at very low levels or not detectable in most adult tissues. Detected in adult heart, placenta, lung, lymph node, spleen and ovary, and in several carcinoma cell lines. Isoform 2 is detected in fetal kidney, heart and spleen, and at lower levels in adult brain, skeletal muscle and peripheral blood leukocytes.

Similarity:
Belongs to the IAP family.
Contains 1 BIR repeat.
Contains 1 RING-type zinc finger.

SWISS:
Q96CA5

Gene ID:
79444

Database links:

Entrez Gene: 79444 Human

Omim: 605737 Human

SwissProt: Q96CA5 Human

Unigene: 256126 Human

Livin—属于凋亡蛋白抑制因子家族（inhibitor of apoptosis proteins，IAP）
Livin是凋亡抑制蛋白家族的新成员，主要通过抑制Caspases蛋白发挥抑制细胞凋亡的作用,主要在胎盘组织中表达，同时在一些肿瘤组织中呈高表达，尤其是在黑色素瘤细胞系表达水平最高。
经研究表明，Livin与肿瘤的发生、发展关系密切,是一个有潜在价值的肿瘤标志物，此外，反义Livin以及Livin活性抑制物的研究有望成为肿瘤治疗的新靶点。 Picture

Paraformaldehyde-fixed, paraffin embedded (Rat skin); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Livin) Polyclonal Antibody, Unconjugated (SL20731R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Blank control (blue line): Hep G2 (fixed with 70% ethanol (Overmight at 4℃) and then permeabilized with 90% ice-cold methanol for 30 min at -20℃).
Primary Antibody (green line): Rabbit Anti-Nanog antibody (SL20731R),Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE,Dilution: 1μg /test.

Blank control: HepG2.
Primary Antibody (green line): Rabbit Anti-Livin antibody (SL20731R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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