Sample:
Lane 1: Spleen (Mouse) Lysate at 40 ug
Lane 2: Lymph node (Mouse) Lysate at 40 ug
Lane 3: Thymus (Mouse) Lysate at 40 ug
Lane 4: Raw264.7 (Mouse) Cell Lysate at 30 ug
Lane 5: Sp2/0 (Mouse) Cell Lysate at 30 ug
Primary: Anti-B7-1/CD80 (SL23296R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 60-65 kD
Observed band size: 62 kD
Sample:
Lane 1: Thymus (Mouse) Lysate at 40 ug
Lane 2: Raw264.7 (Mouse) Cell Lysate at 30 ug
Lane 3: SP2/0 (Mouse) Cell Lysate at 30 ug
Lane 4: Thymus (Rat) Lysate at 40 ug
Lane 5: Raji (Human) Cell Lysate at 30 ug
Lane 6: K562 (Human) Cell Lysate at 30 ug
Primary: Anti-B7-1/CD80 (SL23296R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 60-65 kD
Observed band size: 60 kD