Home > Product > Antibody > Rabbit Anti-B7-1/CD80 antibody
Activation B7-1 antigen; B lymphocyte activation antigen B7; B7; B7-1; B7-1 antigen; BB1; CD28 antigen ligand 1; CD28LG; CD28LG1; CD80; CD80 antigen (CD28 antigen ligand 1, B7-1 antigen); CD80 antigen; CD80 molecule; CD80_MOUSE; Costimulatory factor CD80;
Cat:
SL23296R
Species Reactivity:
Mouse,Rat,
Immunogen:
KLH conjugated synthetic peptide derived from mouse B7-1/CD80:1-100/306<Extracellular>
Format:
Liquid
Storage instructions:
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration:
1mg/ml
Clonality:
Polyclonal
Isotype:
IgG
Applications:
WB=1:500-1000ELISA=1:5000-10000not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host:
Rabbit
Product Overview:
Sample: Lane 1: Spleen (Mouse) Lysate at 40 ugLane 2: Lymph node (Mouse) Lysate at 40 ugLane 3: Thymus (Mouse) Lysate at 40 ugLane 4: Raw264.7 (Mouse) Cell Lysate at 30 ugLane 5: Sp2/0 (Mouse) Cell Lysate at 30 ugPrimary: Anti-B7-1/CD80 (SL23296R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 60-65 kDObserved band size: 62 kDSample: Lane 1: Thymus (Mouse) Lysate at 40 ugLane 2: Raw264.7 (Mouse) Cell Lysate at 30 ugLane 3: SP2/0 (Mouse) Cell Lysate at 30 ugLane 4: Thymus (Rat) Lysate at 40 ugLane 5: Raji (Human) Cell Lysate at 30 ugLane 6: K562 (Human) Cell Lysate at 30 ugPrimary: Anti-B7-1/CD80 (SL23296R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 60-65 kDObserved band size: 60 kD
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Unit:
Price: $
Product PDFs
Datasheet:


CD80 is a member of the Ig superfamily and serves as the ligand for two T cell molecules, CD28 and CTLA4. Interactions between CD28 and CD80 on activated B cells result in enhanced T cell activation. CD80 is rapidly induced on the surface of in vitro activated B cells, Epstein Barr Virus (EBV) transformed B cell lines, Burkitts lymphoma cell lines, freshly isolated follicular B lymphoma cells, T cells, and monocytes. It is also expressed at high levels in dendritic cells. It reacts weakly with a small proportion of non activated normal B cells and with HTLV1 infected T cells. CD80 does not react with peripheral monocytes, resting and activated normal T cells, T cell lines and T cell clones, nor with myelomonocytic cell lines.

Function:
Involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor.

Subcellular Location:
Membrane.

Tissue Specificity:
Expressed on activated B-cells, macrophages and dendritic cells.

Similarity:
Contains 1 Ig-like C2-type (immunoglobulin-like) domain. Contains 1 Ig-like SLVtype (immunoglobulin-like) domain.

SWISS:
Q00609

Gene ID:
12519

Database links:

Entrez Gene: 941 Human

Entrez Gene: 12519 Mouse

Omim: 112203 Human

SwissProt: P33681 Human

SwissProt: Q00609 Mouse

Unigene: 838 Human

Unigene: 89474 Mouse



Picture

Sample:
Lane 1: Spleen (Mouse) Lysate at 40 ug
Lane 2: Lymph node (Mouse) Lysate at 40 ug
Lane 3: Thymus (Mouse) Lysate at 40 ug
Lane 4: Raw264.7 (Mouse) Cell Lysate at 30 ug
Lane 5: Sp2/0 (Mouse) Cell Lysate at 30 ug
Primary: Anti-B7-1/CD80 (SL23296R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 60-65 kD
Observed band size: 62 kD
Sample:
Lane 1: Thymus (Mouse) Lysate at 40 ug
Lane 2: Raw264.7 (Mouse) Cell Lysate at 30 ug
Lane 3: SP2/0 (Mouse) Cell Lysate at 30 ug
Lane 4: Thymus (Rat) Lysate at 40 ug
Lane 5: Raji (Human) Cell Lysate at 30 ug
Lane 6: K562 (Human) Cell Lysate at 30 ug
Primary: Anti-B7-1/CD80 (SL23296R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 60-65 kD
Observed band size: 60 kD
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