Rabbit Anti-ASNS antibody | Sunlong Medical

Home > Product > Antibody > Rabbit Anti-ASNS antibody

Asparagine synthetase; ASNS_HUMAN; Asparagine synthetase [glutamine-hydrolyzing]; TS11; EC:6.3.5.4; Cell cycle control protein TS11; Glutamine-dependent asparagine synthetase; ASNSD;

Cat:

SL23432R

Species Reactivity：

Human,Mouse,(predicted: Dog,Pig,Horse,)

Immunogen：

KLH conjugated synthetic peptide derived from human ASNS:451-550/561

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Pancreas (Mouse) Lysate at 40 ugPrimary: Anti-Asparagine synthetase (SL23432R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 61 kDObserved band size: 61 kDSample: K562(Human) Cell Lysate at 30 ugMCF-7(Human) Cell Lysate at 30 ugPrimary: Anti-Asparagine synthetase (SL23432R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 61 kDObserved band size: 63 kD Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Asparagine synthetase) Polyclonal Antibody, Unconjugated (SL23432R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

prev next

Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
Feedback Wall

The protein encoded by this gene is involved in the synthesis of asparagine. This gene complements a mutation in the temperature-sensitive hamster mutant ts11, which blocks progression through the G1 phase of the cell cycle at nonpermissive temperature. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, May 2010]

DISEASE:
Asparagine synthetase deficiency (ASNSD) [MIM:615574]: An inborn error of asparagine biosynthesis that results in a severe neurologic disorder characterized by microcephaly, severely delayed psychomotor development, progressive encephalopathy, cortical atrophy, and seizure or hyperekplexic activity. {ECO:0000269|PubMed:24139043}. Note=The disease is caused by mutations affecting the gene represented in this entry.

Similarity:
Contains 1 asparagine synthetase domain.
Contains 1 glutamine amidotransferase type-2 domain.

SWISS:
P08243

Gene ID:
88

Database links:

Entrez Gene: 88 Human

Entrez Gene: 27053 Mouse

Entrez Gene: 25612 Rat

SwissProt: P08243 Human

SwissProt: Q61024 Mouse

SwissProt: P49088 Rat

Picture

Sample:
K562(Human) Cell Lysate at 30 ug
MCF-7(Human) Cell Lysate at 30 ug
Primary: Anti-Asparagine synthetase (SL23432R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 61 kD
Observed band size: 63 kD

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Asparagine synthetase) Polyclonal Antibody, Unconjugated (SL23432R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Product Feedback Wall