Rabbit Anti-GCLC antibody | Sunlong Medical

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Gamma ECS; Gamma GCS; Gamma glutamylcysteine synthetase; Gamma-ECS; Gamma-glutamylcysteine synthetase; GCL; GCLC; GCS; GCS Heavy Chain antibody GLCL; GLCLC; GLCLR; Glutamate cysteine ligase catalytic subunit; Glutamate cysteine ligase (gamma glutamylcyste

Cat:

SL23393R

Species Reactivity：

Mouse,Rat,(predicted: Human,Chicken,Dog,Pig,Cow,Horse,Rabbit,Zebrafish,)

Immunogen：

KLH conjugated synthetic peptide derived from human GCLC:351-450/637

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Liver (Mouse) Lysate at 40 ugPrimary: Anti-GCLC (SL23393R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 73 kDObserved band size: 73 kDSample: Kidney (Mouse) Lysate at 40 ugKidney (Rat) Lysate at 40 ugPrimary: Anti-GCLC (SL23393R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 73 kDObserved band size: 73 kD Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GCLC) Polyclonal Antibody, Unconjugated (SL23393R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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The GCLC gene consists of 16 exons and encodes the 636 amino acid protein g-GCSc (g-glutamylcysteine synthetase heavy subunit), also designated g-L-glutamate-L-cysteine ligase catalytic subunit (GLCLC). g-GCSc is expressed in hemocytes, brain, liver and kidney. g-GCSc associates with a regulatory or modifier subunit, g-GCSm (g-glutamylcysteine synthetase light subunit), to form a heterodimer, g-GCS. g-GCS is the first enzyme involved and the rate determining step in glutathione biosynthesis. Oxidants, cadium and methyl mercury upregulate the transcription of g-GCS. H2O2 regulation depends on the Yap1 protein and the presence of glutamate, glutamine and lysine. Cadium regulates transcription through proteins Met-4, Met-31 and Met-32. Cbf1, a DNA binding protein, inhibits transcription of g-GCS. Chemopreventive compounds cause increased levels of g-GCSc in kidney tissues, which may protect against chemically induced carcinogenesis. A His370Leu amino acid change in g-GCSc causes deficiencies in activity which are responsible for hemolytic anemia and low red blood cell glutathione levels. Defects in GCLC are the cause of hemolytic anemia.

Subunit:
Heterodimer of a catalytic heavy chain and a regulatory light chain.

DISEASE:
Defects in GCLC are the cause of hemolytic anemia (HAGGSD) [MIM:230450].

Similarity:
Belongs to the glutamate--cysteine ligase type 3 family.

SWISS:
P48506

Gene ID:
2729

Database links:

Entrez Gene: 2729 Human

Entrez Gene: 14629 Mouse

Entrez Gene: 25283 Rat

Omim: 606857 Human

SwissProt: P48506 Human

SwissProt: P97494 Mouse

SwissProt: P19468 Rat

Unigene: 654465 Human

Unigene: 89888 Mouse

Unigene: 8365 Rat

Picture

Sample:
Kidney (Mouse) Lysate at 40 ug
Kidney (Rat) Lysate at 40 ug
Primary: Anti-GCLC (SL23393R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 73 kD
Observed band size: 73 kD

Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GCLC) Polyclonal Antibody, Unconjugated (SL23393R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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