This gene is a member of the NAD(P)H dehydrogenase (quinone) family and encodes a cytoplasmic 2-electron reductase. This FAD-binding protein forms homodimers and reduces quinones to hydroquinones. This protein's enzymatic activity prevents the one electron reduction of quinones that results in the production of radical species. Mutations in this gene have been associated with tardive dyskinesia (TD), an increased risk of hematotoxicity after exposure to benzene, and susceptibility to various forms of cancer. Altered expression of this protein has been seen in many tumors and is also associated with Alzheimer's disease (AD). Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq].
Function:
The enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis.
Subunit:
Homodimer.
Subcellular Location:
Cytoplasm.
Similarity:
Belongs to the NAD(P)H dehydrogenase (quinone) family.
SWISS:
Q64669
Gene ID:
18104
Database links:
Entrez Gene: 610935 Dog
Entrez Gene: 1728 Human
Entrez Gene: 18104 Mouse
Entrez Gene: 100286873 Pig
Entrez Gene: 24314 Rat
Omim: 125860 Human
SwissProt: P15559 Human
SwissProt: Q64669 Mouse
SwissProt: P05982 Rat
Unigene: 406515 Human
Unigene: 252 Mouse
Unigene: 11234 Rat
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Sample:
Siha(Human) Cell Lysate at 30 ug
Primary: Anti-NQO1 (SL23407R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 31 kD
Observed band size: 31 kD
Sample:
Stomach (Mouse) Lysate at 40 ug
Primary: Anti-NQO1 (SL23407R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 31 kD
Observed band size: 31 kD
Sample:
A549(Human) Cell Lysate at 30 ug
Primary: Anti-NQO1 (SL23407R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 31 kD
Observed band size: 31 kD
Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NQO1) Polyclonal Antibody, Unconjugated (SL23407R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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