Rabbit Anti-CD80 antibody | Sunlong Medical

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Activation B7-1 antigen; B lymphocyte activation antigen B7; B7; B7-1; B7-1 antigen; BB1; CD28 antigen ligand 1; CD28LG; CD28LG1; CD80 antigen (CD28 antigen ligand 1, B7-1 antigen); CD80 antigen; CD80 molecule; CD80_HUMAN; Costimulatory factor CD80; costi

Cat:

SL23738R

Species Reactivity：

Human,

Immunogen：

KLH conjugated synthetic peptide derived from human CD80:201-300/288<Extracellular>

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: U937(Human) Cell Lysate at 30 ugPrimary: Anti-CD80 (SL23738R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 32 kDObserved band size: 32 kDSample: Raji(Human) Cell Lysate at 30 ugPrimary: Anti-CD80 (SL23738R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 32 kDObserved band size: 32 kDParaformaldehyde-fixed, paraffin embedded (Human melanoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD80) Polyclonal Antibody, Unconjugated (SL23738R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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CD80 is a member of the Ig superfamily and serves as the ligand for two T cell molecules, CD28 and CTLA4. Interactions between CD28 and CD80 on activated B cells result in enhanced T cell activation. CD80 is rapidly induced on the surface of in vitro activated B cells, Epstein Barr Virus (EBV) transformed B cell lines, Burkitts lymphoma cell lines, freshly isolated follicular B lymphoma cells, T cells, and monocytes. It is also expressed at high levels in dendritic cells. It reacts weakly with a small proportion of non activated normal B cells and with HTLV1 infected T cells. CD80 does not react with peripheral monocytes, resting and activated normal T cells, T cell lines and T cell clones, nor with myelomonocytic cell lines.

Function:
Involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor.

Subcellular Location:
Membrane.

Tissue Specificity:
Expressed on activated B-cells, macrophages and dendritic cells.

Similarity:
Contains 1 Ig-like C2-type (immunoglobulin-like) domain. Contains 1 Ig-like SLVtype (immunoglobulin-like) domain.

SWISS:
P33681

Gene ID:
941

Database links:

Entrez Gene: 941 Human

Entrez Gene: 12519 Mouse

Omim: 112203 Human

SwissProt: P33681 Human

SwissProt: Q00609 Mouse

Unigene: 838 Human

Unigene: 89474 Mouse

Picture

Sample:
Raji(Human) Cell Lysate at 30 ug
Primary: Anti-CD80 (SL23738R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 32 kD
Observed band size: 32 kD

Paraformaldehyde-fixed, paraffin embedded (Human melanoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD80) Polyclonal Antibody, Unconjugated (SL23738R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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