Rabbit Anti-Artemin antibody

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Artemin; ARTN; Enovin; EVN; NBN; Neublastin

Cat:

SL22203R

Species Reactivity：

Mouse,Rat,(predicted: Human,)

Immunogen：

KLH conjugated synthetic peptide derived from mouse Artemin:141-224/224

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Testis (Mouse) Lysate at 40 ugPrimary: Anti- Artemin (SL22203R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 12/19 kDObserved band size: 19 kDParaformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Artemin) Polyclonal Antibody, Unconjugated (SL22203R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Artemin) Polyclonal Antibody, Unconjugated (SL22203R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Artemin is a novel member of the GDNF ligand family, supports peripheral and central neurons and signals through the GFR alpha3-RET receptor complex. The protein encoded by this gene is a member of the glial cell line-derived neurotophic factor (GDNF) family of ligands which are a group of ligands within the TGF-beta superfamily of signaling molecules. GDNFs are unique in having neurotrophic properties and have potential use for gene therapy in neurodegenrative disease. Artemin has been shown in culture to support the survival of a number of periferal neuron populations and at least one population of dopaminergic CNS neurons. Its role in the PNS and CNS is further substantiated by its expression pattern in the proximity of these neurons. This protein is a ligand for the RET receptor and uses GFR-alpha 3 as a coreceptor. Four alternatively spliced transcripts have been described, two of which encode the same protein.

Function:
Ligand for the GFR-alpha-3-RET receptor complex but can also activate the GFR-alpha-1-RET receptor complex. Supports the survival of sensory and sympathetic peripheral neurons in culture and also supports the survival of dopaminergic neurons of the ventral mid-brain. Strong attractant of gut hematopoietic cells thus promoting the formation Peyer's patch-like structures, a major component of the gut-associated lymphoid tissue (By similarity). Subunit : Homodimer; disulfide-linked. Binds to RET.

Subcellular Location:
Secreted.

Tissue Specificity:
Cochlea. Expressed at higher level in sesorineural epithelium than in the modiolus region or substantia nigra.

Similarity:
Belongs to the TGF-beta family. GDNF subfamily.

SWISS:
Q9Z0L2

Gene ID:
11876

Database links:
Entrez Gene: 9048 Human

Entrez Gene: 11876 Mouse

Entrez Gene: 362572 Rat

Omim: 603886 Human

SwissProt: Q5T4W7 Human

SwissProt: Q9Z0L2 Mouse

SwissProt: Q6AYE8 Rat

Unigene: 63484 Human

Unigene: 56897 Mouse

Unigene: 154711 Rat

神经生物学相关蛋白（Neurobiology） Artemin 是GDNF 家族的一个新成员，已被证实它是从前的孤儿受体GFRalpha3-RET的配体。 Artemin是感觉和交感神经元培养的一个存活因子，它的表达形式也影响体内的这些神经元。Artemin 还能激活GFRalpha1-RET 复合物并支持培养中脑多巴胺神经元存活 Picture

Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Artemin) Polyclonal Antibody, Unconjugated (SL22203R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Artemin) Polyclonal Antibody, Unconjugated (SL22203R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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