Rabbit Anti-PCSK1/NEC1 antibody

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BDP; NEC 1; NEC1; NEC1_HUMAN; Neuroendocrine convertase 1; PC1; PC3; PCSK1; Prohormone convertase 1; Prohormone convertase 1/3; Prohormone convertase 3; Proprotein convertase 1; SPC3.

Cat:

SL24307R

Species Reactivity：

Human,Mouse,(predicted: Rat,Chicken,Pig,Cow,Horse,Rabbit,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human PCSK1/NEC1:311-410/753

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Spleen (Mouse) Lysate at 40 ugPrimary: Anti-PCSK1’NEC1 (SL24307R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 71 kDObserved band size: 71 kDSample: Hela (Human) Cell Lysate at 30 ugPrimary: Anti- PCSK1/NEC1 (SL24307R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 71 kDObserved band size: 75 kDParaformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PCSK1/NEC1) Polyclonal Antibody, Unconjugated (SL24307R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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This gene encodes a member of the subtilisin-like proprotein convertase family, which includes proteases that process protein and peptide precursors trafficking through regulated or constitutive branches of the secretory pathway. The encoded protein undergoes an initial autocatalytic processing event in the ER to generate a heterodimer which exits the ER and sorts to subcellular compartments where a second autocatalytic even takes place and the catalytic activity is acquired. The protease is packaged into and activated in dense core secretory granules and expressed in the neuroendocrine system and brain. This gene encodes one of the seven basic amino acid-specific members which cleave their substrates at single or paired basic residues. It functions in the proteolytic activation of polypeptide hormones and neuropeptides precursors. Mutations in this gene have been associated with susceptibility to obesity and proprotein convertase 1/3 deficiency. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene [provided by RefSeq, Jan 2014]

Function:
Involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. Substrates include POMC, renin, enkephalin, dynorphin, somatostatin, insulin and AGRP.

Subcellular Location:
Cytoplasmic vesicle > secretory vesicle. Localized in the secretion granules.

DISEASE:
Defects in PCSK1 are the cause of proprotein convertase 1 deficiency (PC1 deficiency) [MIM:600955]. PC1 deficiency is characterized by obesity, hypogonadism, hypoadrenalism, reactive hypoglycemia as well as marked small-intestinal absorptive dysfunction It is due to impaired processing of prohormones.

Similarity:
Belongs to the peptidase S8 family. Furin subfamily.

SWISS:
P29120

Gene ID:
5122

Database links:

Entrez Gene: 281967 Cow

Entrez Gene: 5122 Human

Entrez Gene: 18548 Mouse

Entrez Gene: 397103 Pig

Entrez Gene: 25204 Rat

Omim: 162150 Human

SwissProt: Q9GLR1 Cow

SwissProt: P29120 Human

SwissProt: P63239 Mouse

SwissProt: P28168 Rat

Unigene: 78977 Human

Unigene: 1333 Mouse

Unigene: 2284 Rat

Picture

Sample:
Hela (Human) Cell Lysate at 30 ug
Primary: Anti- PCSK1/NEC1 (SL24307R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 71 kD
Observed band size: 75 kD

Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PCSK1/NEC1) Polyclonal Antibody, Unconjugated (SL24307R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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