Rabbit Anti-F-Actin antibody

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f actin; Filamentous actin; F-actin capping protein alpha subunit; CapZ alpha-1; CAZA1_HUMAN; CAPZA1.

Cat:

SL1571R

Species Reactivity：

Human,Mouse,Rat,(predicted: Pig,Cow,Sheep,)

Immunogen：

Synthetic MAP peptide derived from human beta-Actin:1-50/374

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:1000-5000ELISA=1:5000-10000IHC-P=1:100-500Flow-Cyt=3μg /test（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Lane1:Hela (Human) Whole Cell Lysate at 30 ugLane2:Jurkat (Human) Whole Cell Lysate at 30 ugLane3:293T (Human) Whole Cell Lysate at 30 ugLane4:Hepg2 (Human) Whole Cell Lysate at 30 ugLane5:A431 (Human) Whole Cell Lysate at 30 ugLane6:A549 (Human) Whole Cell Lysate at 30 ugLane7:H9C2 (Rat) Whole Cell Lysate at 30 ugLane8:Testis (Mouse) Tissue Lysate at 30 ugPrimary: Anti-F-Actin(SL1571R)at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 42 kDObserved band size: 42 kDParaformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (F-Actin) Polyclonal Antibody, Unconjugated (SL1571R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (F-Actin) Polyclonal Antibody, Unconjugated (SL1571R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Blank control (blue line): Hela (blue). Primary Antibody (green line): Rabbit Anti- F-Actin antibody (SL1571R) Dilution: 3μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-PEDilution: 1μg /test. ProtocolThe cells were fixed with 80% methanol (5 min at -20℃) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature . Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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In vertebrates, three main groups of actin isoforms, alpha, beta, and gamma have been identified. The alpha actins, found in muscletissues, are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility. Individual subunits of microfilaments are known as globular actin (G-actin). G-actin subunits assemble into long filamentous polymers called F-actin.

SWISS:
P60709

Gene ID:
60

Database links:

Entrez Gene: 60 Human

Entrez Gene: 11461 Mouse

Entrez Gene: 100009272 Rabbit

Entrez Gene: 81822 Rat

Omim: 102630 Human

SwissProt: P60709 Human

SwissProt: P60710 Mouse

SwissProt: P29751 Rabbit

SwissProt: P60711 Rat

Unigene: 520128 Human

Unigene: 708120 Human

Unigene: 727576 Human

Unigene: 328431 Mouse

Unigene: 391967 Mouse

Unigene: 94978 Rat

纤维状肌动蛋白F-Actin主要存在于横纹肌原肌纤维细丝及平滑肌中，是肌肉运动和细胞运动的重要蛋白。 Picture

Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (F-Actin) Polyclonal Antibody, Unconjugated (SL1571R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (F-Actin) Polyclonal Antibody, Unconjugated (SL1571R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Blank control (blue line): Hela (blue).
Primary Antibody (green line): Rabbit Anti- F-Actin antibody (SL1571R)
Dilution: 3μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 80% methanol (5 min at -20℃) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature . Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

Product Feedback Wall

Specific References (30) | SL1571R has been referenced in 30 publications.

[IF=2.78] Jing et al. Expression of immunoglobulin G in human podocytes, and its role in cell viability and adhesion. (2018) Int.J.Mol.Med. 41:3296-3306 IF ; Human.

PubMed:29512722

[IF=2.766] Bwalya et al. Pentosan polysulfate inhibits IL-1β-induced iNOS, c-Jun and HIF-1α upregulation in canine articular chondrocytes. (2017) PLoS.One. 12:e0177144 WB ; Dog.

PubMed:28472120

[IF=4.556] Tomoko Okada. et al. Scanning Electron-Assisted Dielectric Microscopy Reveals Autophagosome Formation by LC3 and ATG12 in Cultured Mammalian Cells. Int J Mol Sci. 2021 Jan;22(4):1834 IF ; Rat.

PubMed:33673233

[IF=4.011] Shiozawa M et al. Biological reaction control using topography regulation of nanostructured titanium. Sci Rep. 2020 Feb 12;10(1):2438. ICF ; Rat.

PubMed:6451472

[IF=0.88] Murakami et al. Roles of Ras Homolog A in Invasive Ductal Breast Carcinoma. (2016) Acta.Histochem.Cytochem. 49:131-140 IHC ; Human.

PubMed:27917007

[IF=2.22] Li, Haihong, et al. "Human eccrine sweat gland cells reconstitute polarized spheroids when subcutaneously implanted with Matrigel in nude mice." Journal of molecular histology 47.5 (2016): 485-490. IHSLCP ; Mouse.

PubMed:27492422

[IF=2.52] Chen, S. M., et al. "Cofilin is correlated with sperm quality and influences sperm fertilizing capacity in humans." Andrology (2016). WB ; Human.

PubMed:27369112

[IF=0.18] Du, Ailin, et al. "Damage of hippocampal neurons in rats with chronic alcoholism." Neural regeneration research 9.17 (2014): 1610. IHSLCP ; Rat.

PubMed:25368648

[IF=0] Deguchi, Takahiro. "Development of 3D super-resolution tomographic STED microscopy and its application to studies on bone resorption." (2015). other ;

PubMed:ISBN978-951-29-6317-1

[IF=0.86] Xie, Chaoming, Xiong Lu, and Kefeng Wang. "Pulse Electrochemical Synthesis of Spherical Hydroxyapatite and Silver Nanoparticles Mediated by the Polymerization of Polypyrrole on Metallic Implants for Biomedical Applications." Particle & Particle Systems Characterization (2015). Rat.

PubMed:10.1002/ppsc.201400245

[IF=2.8] Kang, Hongyan, et al. "A Potential Gravity-Sensing Role of Vascular Smooth Muscle Cell Glycocalyx in Altered Gravitational Stimulation." Astrobiology (2013). WB ; Rat.

PubMed:23848471

[IF=1.86] Siar, Chong Huat, et al. "Invadopodia proteins, cortactin, N‐WASP and WIP differentially promote local invasiveness in ameloblastoma." Journal of Oral Pathology & Medicine (2016). IHSLCP ; Human.

PubMed:26752341

[IF=1.69] Guo, Xiliang, and Xuezheng Liu. "Nogo receptor knockdown and ciliary neurotrophic factor attenuate diabetic retinopathy in streptozotocin-induced diabetic rats." Molecular Medicine Reports. (2017). IHSLCP ; Rat.

PubMed:28656312

[IF=3.22] Zhong, Weiliang, et al. "An integrated microfluidic device for characterizing chondrocyte metabolism in response to distinct levels of fluid flow stimulus."Microfluidics and Nanofluidics: 1-11.

PubMed:10.1007/s10404-013-1186-9

[IF=3.73] Zhong, Weiliang, et al. "Regulation of Fibrochondrogenesis of Mesenchymal Stem Cells in an Integrated Microfluidic Platform Embedded with Biomimetic Nanofibrous Scaffolds." PLOS ONE 8.4 (2013): e61283. Rat.

PubMed:23637803

[IF=6.126] Taketo Suzuki. et al. Vascular Shutdown by Photodynamic Therapy Using Talaporfin Sodium. Cancers. 2020 Sep;12(9):2369 ICC ; Human.

PubMed:32825648

[IF=2.788] Alisha Wehdnesday Bernard Reyes. et al. Formyl peptide receptor 2 (FPR2) antagonism is a potential target for the prevention of Brucella abortus 544 infection. Immunobiology. 2021 May;226:152073 WB ; Mouse.

PubMed:33657463

[IF=4.421] Bareera Qamar. et al. Intracellular Delivery of Active Proteins by Polyphosphazene Polymers. Pharmaceutics. 2021 Feb;13(2):249 ICC ; Human.

PubMed:33578893

[IF=3.73] Su, Bao, et al. "In Vitro and In Vivo Evaluations of Nano-Hydroxyapatite/Polyamide 66/Glass Fibre (n-HA/PA66/GF) as a Novel Bioactive Bone Screw." PLOS ONE 8.7 (2013): e68342. Mouse.

PubMed:23861888

[IF=0] Okada T et al. High-resolution imaging of autophagosome formation by LC3 and ATG12 in cells using scanning electron-assisted dielectric microscopy. Research Square; 2020 Feb 7. ICF ; Mouse&Rat.

PubMed:doi:10.21203/rs.2.22874/v1

[IF=5.391] Yagi H et al. Gα13-mediated LATS1 down-regulation contributes to epithelial-mesenchymal transition in ovarian cancer. FASEB J. 2019 Sep 28:fj201901278R. ICF ; Human.

PubMed:31569999

[IF=2.559] Li X et al. LncRNA FTX inhibits hippocampal neuron apoptosis by regulating miR-21-5p/SOX7 axis in a rat model of temporal lobe epilepsy.Biochem Biophys Res Commun. 2019 Apr 23;512(1):79-86. WB ; Rat.

PubMed:30871773

[IF=2.8] Zhai J et al. An increase of estrogen receptor α protein level regulates BDE-209-mediated blood-testis barrier disruption during spermatogenesis in F1 mice. (2018) Environ Sci Pollut Res Int. WB,IHF&ICF ; Mouse.

PubMed:30565106

[IF=2.766] Okada T et al. Nanoscale imaging of the adhesion core including integrin β1 on intact living cells using scanning electron-assisted dielectric-impedance microscopy. (2018) PLOS ONE, 13(9), e0204133. IF，ICC ; Mouse.

PubMed:30235285

[IF=0] Bwalya E et al. Pentosan Polysulfate Sodium Restores the Phenotype of Dedifferentiated Monolayer Canine Articular Chondrocytes Cultured in Alginate Bea. J Tissue Sci Eng 2018, 9:1. WB ; Dog.

PubMed:DOI:10.4172/2157-7552.1000218

[IF=2.74] Clark et al. The human, F-actin-based cytoskeleton as a mutagen sensor. (2017) Cancer.Cell.Int. 17:121 IF(IHSLCP) ; Human.

PubMed:29255378

[IF=5.79] Yang et al. Rab5-mediated VE-cadherin internalization regulates the barrier function of the lung microvascular endothelium. (2015) Cell.Mol.Life.Sci. 72:4849-66 WB,ICC ; Human.

PubMed:26112597

[IF=2.74] Clark, Nicolette M., et al. "The human, F-actin-based cytoskeleton as a mutagen sensor." Cancer Cell International 17.1 (2017): 121. IHSLCP ; Human.

PubMed:29255378

[IF=1.84] Zhang, Pengfei, et al. "Proteome analysis of egg yolk after exposure to zinc oxide nanoparticles." Theriogenology (2017). WB ; Chicken.

PubMed:2892670

[IF=2.55] Zhao, Ran, et al. "Hepatoma cell line HepG2. 2.15 demonstrates distinct biological features compared with parental HepG2." World journal of gastroenterology: WJG 17.9 (2011): 1152. WB ; Human.

PubMed:21448419