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Home > Product > Antibody > Rabbit Anti-SIGLEC1 antibody
CD 169; CD-169; dJ1009E24.1; DKFZp667F058; FLJ00051; FLJ00055; FLJ00073; FLJ00411; FLJ32150; Sialic acid binding Ig like lectin 1; Sialic acid binding immunoglobulin like lectin 1; Siglec 1; SN; sialoadhesin; SN_HUMAN.
Cat:
SL0794R
Species Reactivity：
Human,Mouse,(predicted: Rat,)
Immunogen：
KLH conjugated synthetic peptide derived from human SIGLEC1:51-150/1694<Extracellular>
Format：
Liquid
Storage instructions：
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration：
1mg/ml
Clonality：
Polyclonal
Isotype：
IgG
Applications：
IHC-P=1:100-500IHC-F=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host：
Rabbit
Product Overview：
Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Sialoadhesin) Polyclonal Antibody, Unconjugated (SL0794R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-CD169/sialoadhesin Polyclonal Antibody, Unconjugated(SL0794R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
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Product PDFs
Datasheet:


This gene encodes a member of the immunoglobulin superfamily. The encoded protein is a lectin-like adhesion molecule that binds glycoconjugate ligands on cell surfaces in a sialic acid-dependent manner. It is a type I transmembrane protein expressed only by a subpopulation of macrophages and is involved in mediating cell-cell interactions. Alternative splicing produces a transcript variant encoding an isoform that is soluble rather than membrane-bound; however, the full-length nature of this variant has not been determined. [provided by RefSeq, Jul 2008].

Function:
Acts as an endocytic receptor mediating clathrin dependent endocytosis. Macrophage-restricted adhesion molecule that mediates sialic-acid dependent binding to lymphocytes, including granulocytes, monocytes, natural killer cells, B-cells and CD8 T-cells. Preferentially binds to alpha-2,3-linked sialic acid. Binds to SPN/CD43 on T-cells. May play a role in hemopoiesis.

Subunit:
Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2: Secreted.

Subcellular Location:
Expressed by macrophages in various tissues. High levels are found in spleen, lymph node, perivascular macrophages in brain and lower levels in bone marrow, liver Kupffer cells and lamina propria of colon and lung. Also expressed by inflammatory macrophages in rheumatoid arthritis.

Similarity:
Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
Contains 16 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like SLVtype (immunoglobulin-like) domain.

SWISS:
Q9BZZ2

Gene ID:
6614

Database links:

Entrez Gene: 6614 Human

Entrez Gene: 20612 Mouse

Omim: 600751 Human

SwissProt: Q9BZZ2 Human

SwissProt: Q62230 Mouse

Unigene: 31869 Human

Unigene: 1374 Mouse



CD169，又称sialoadhesin（Sn）或Siglee-1，这是一种200kDa的I型跨膜糖蛋白，由炎性巨噬细胞特异性表达，在细胞-细胞或细胞-基质的相互作用中发挥主要作用。可识别乳癌细胞的MUC1（CD227）和T细胞的CD43。CD169在脾、淋巴结、骨髓的基质巨噬细胞上表达最强，在肝、肠、肺的长驻巨噬细胞上中等表达，慢性炎组织的巨噬细胞，例如类风湿性滑膜、动脉粥样硬化性斑块，也强烈表达CD169。 Picture

Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Sialoadhesin) Polyclonal Antibody, Unconjugated (SL0794R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-CD169/sialoadhesin Polyclonal Antibody, Unconjugated(SL0794R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
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