Rabbit Anti-CD36 antibody | Sunlong Medical

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Platelet glycoprotein 4; PAS-4; Platelet glycoprotein IV; Glycoprotein IIIb; GPIV; GPIIIB; GP3B; Leukocyte differentiation antigen CD36; PAS IV; Platelet collagen receptor; Fatty acid translocase; FAT; Thrombospondin receptor; CD_antigen: GP4; CD36_HUMAN.

Cat:

SL1100R

Species Reactivity：

Human,Mouse,Rat,(predicted: Dog,Pig,Cow,Horse,)

Immunogen：

KLH conjugated synthetic peptide derived from mouse CD36:51-150/472<Extracellular>

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Lane 1: Mouse Placenta tissue lysates Lane 2: Mouse Raw264.7 cell lysates Lane 3: Rat Placenta tissue lysatesLane 4: Human HUVEC cell lysates Lane 5: Human HL-60 cell lysates Primary: Anti-CD36 (SL1100R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 52 kDaObserved band size: 100 kDaSample: Lane 1: Human HepG2 cell lysates Lane 2: Human HUVEC cell lysates Lane 3: Human MCF-7cell lysates Primary: Anti-CD36 (SL1100R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 52 kDaObserved band size: 90 kDaTissue/cell: rat heart tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-CD36/PAS-4 Polyclonal Antibody, Unconjugated(SL1100R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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The protein encoded by this gene is the fourth major glycoprotein of the platelet surface and serves as a receptor for thrombospondin in platelets and various cell lines. Since thrombospondins are widely distributed proteins involved in a variety of adhesive processes, this protein may have important functions as a cell adhesion molecule. It binds to collagen, thrombospondin, anionic phospholipids and oxidized LDL. It directly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes and it binds long chain fatty acids and may function in the transport and/or as a regulator of fatty acid transport. Mutations in this gene cause platelet glycoprotein deficiency. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Feb 2014]

Function:
Seems to have numerous potential physiological functions. Binds to collagen, thrombospondin, anionic phospholipids and oxidized LDL. May function as a cell adhesion molecule. Directly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes. Binds long chain fatty acids and may function in the transport and/or as a regulator of fatty acid transport. Receptor for thombospondins, THBS1 AND THBS2, mediating their antiangiogenic effects.

Subunit:
Interacts with THBS1 and THBS2; the interactions mediate the THBS antiangiogenic activity.

Subcellular Location:
Membrane; Multi-pass membrane protein.

Tissue Specificity:
Predominant in heart, intestine, spleen, fat, skeletal muscle, lower in testes.

Post-translational modifications:
N-glycosylated and O-glycosylated with a ratio of 2:1.

DISEASE:
Defects in CD36 are the cause of platelet glycoprotein IV deficiency (PG4D)[MIM:601684]; also known as CD36 deficiency. Platelet glycoprotein IV deficiency can be divided into 2 subgroups. The type I phenotype is characterized by platelets and monocytes/macrophages exhibiting complete CD36 deficiency. The type II phenotype lacks the surface expression of CD36 in platelets, but expression in monocytes/macrophages is near normal.
Genetic variations in CD36 are associated with susceptibility to coronary heart disease type 7 (CHDS7) [MIM:610938].

Similarity:
Belongs to the CD36 family.

SWISS:
Q08857

Gene ID:
12491

Database links:

Entrez Gene: 948 Human

Entrez Gene: 12491 Mouse

Entrez Gene: 29184 Rat

Omim: 173510 Human

SwissProt: P16671 Human

SwissProt: Q08857 Mouse

SwissProt: Q07969 Rat

Unigene: 120949 Human

Unigene: 18628 Mouse

Unigene: 406799 Mouse

Unigene: 102418 Rat

Unigene: 205168 Rat

CD36属一种多功能细胞膜受体，CD36参与长链脂肪酸的吸附和调节细胞凋亡残物的清理和巨噬细胞的吞噬作用，属于一种抗蛋白酶血小板膜表面糖蛋白。
CD36 广泛在单核细胞、巨噬细胞、内皮层细胞、血小板、红细胞前板、脂肪细胞、肌肉细胞以及乳腺内皮层细胞中有不同的表达。CD36抗原是血小板反应素的配体，两者结合后产生抑制肿瘤血管生成的效应。 Picture

Sample:
Lane 1: Human HepG2 cell lysates
Lane 2: Human HUVEC cell lysates
Lane 3: Human MCF-7cell lysates
Primary: Anti-CD36 (SL1100R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52 kDa
Observed band size: 90 kDa

Tissue/cell: rat heart tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-CD36/PAS-4 Polyclonal Antibody, Unconjugated(SL1100R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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