Rabbit Anti-Cytochrome b245 Light Chain antibody

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CYBA; Cytochrome b 245 alpha polypeptide; Cytochrome b 245 light chain; Cytochrome b alpha polypeptide; Cytochrome b(558) alpha chain; Cytochrome b(558) alpha subunit; Cytochrome b558 subunit alpha; Flavocytochrome b 558 alpha polypeptide; Neutrophil cyto

Cat:

SL3879R

Species Reactivity：

Human,Mouse,(predicted: Rat,)

Immunogen：

KLH conjugated synthetic peptide derived from human CK18 Cytochrome b245 Light Chain/p22phox:21-120/195

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Tissue/cell: human liver carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-CYBA Polyclonal Antibody, Unconjugated(SL3879R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingThe blue histogram is unstained cells(mouse Splenocytes). The green histogram is cells stained with Rabbit Anti-Cytochrome b245 Light Chain antibody (SL3879R) plus secondary antibody(SL0295G-FITC). Concentration 1:50.

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Mox1 and the glycoprotein gp91-phox are largely related proteins that are essential components of the NADPH oxidase. The superoxide-generating NADPH oxidase is present in phagocytes, neuroepithelial bodies, vascular smooth muscle cells and endothelial cells. It includes a membrane-bound flavocytochrome containing two subunits, gp91-phox and p22-phox, and the cytosolic proteins p47-phox and p67-phox. During activation of the NADPH oxidase, p47-phox and p67-phox migrate to the plasma membrane, where they associate with the flavocytochrome cytochrome b558 to form the active enzyme complex. The p22- and gp91-phox subunits also function as surface O2 sensors that initiate cellular signaling in response to hypoxic conditions.

Function:
Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. Associates with NOX3 to form a functional NADPH oxidase constitutively generating superoxide.

Subunit:
Composed of a heavy chain (beta) and a light chain (alpha). Interacts with SH3PXD2A (By similarity). Interacts with DUOX1, DUOX2 and TPO. Interacts with NOX3 and NOX4. Interacts with calprotectin (S100A8/9).

Subcellular Location:
Cell Membrane

Post-translational modifications:
The heme prosthetic group could be coordinated with residues of the light chain, the heavy chain, or both, and it is possible that more than one heme is present per cytochrome b-245. [PTM] Phosphorylation at Thr-147 enhances NADPH oxidase activity by promoting p47phox binding (By similarity).

DISEASE:
Granulomatous disease, chronic, cytochrome-b-negative, autosomal recessive (ARCGD) [MIM:233690]: A disorder characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients suffer from life-threatening bacterial/fungal infections. Note=The disease is caused by mutations affecting the gene represented in this entry.

Similarity:
Belongs to the p22phox family.

SWISS:
P13498

Gene ID:
1535

Database links:

Entrez Gene: 1535 Human

Entrez Gene: 13057 Mouse

Entrez Gene: 79129 Rat

Omim: 608508 Human

SwissProt: P13498 Human

SwissProt: Q61462 Mouse

SwissProt: Q62737 Rat

Unigene: 52763 Human

p22phox又称中性粒细胞色素b22kDa的多肽，主要用于心血管病及心肺功能的研究。 Picture

The blue histogram is unstained cells(mouse Splenocytes). The green histogram is cells stained with Rabbit Anti-Cytochrome b245 Light Chain antibody (SL3879R) plus secondary antibody(SL0295G-FITC). Concentration 1:50.

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