The protein encoded by this gene is a member of the G-protein-coupled receptor family. This protein is a receptor for interleukin 8 (IL8). It binds to IL8 with high affinity, and transduces the signal through a G-protein activated second messenger system. Knockout studies in mice suggested that this protein inhibits embryonic oligodendrocyte precursor migration in developing spinal cord. This gene, IL8RB, a gene encoding another high affinity IL8 receptor, as well as IL8RBP, a pseudogene of IL8RB, form a gene cluster in a region mapped to chromosome 2q33-q36. [provided by RefSeq, Jul 2008]
Function:
Receptor to interleukin-8, which is a powerful neutrophils chemotactic factor. Binding of IL-8 to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activate a phosphatidylinositol-calcium second messenger system. This receptor binds to IL-8 with a high affinity and to MGSA (GRO) with a low affinity.
Subcellular Location:
Cell membrane.
Similarity:
Belongs to the G-protein coupled receptor 1 family.
SWISS:
P25024
Gene ID:
3577
Database links:
Entrez Gene: 3577 Human
Entrez Gene: 227288 Mouse
Entrez Gene: 54258 Rat
Omim: 146929 Human
SwissProt: P25024 Human
SwissProt: Q810W6 Mouse
SwissProt: P70612 Rat
Unigene: 194778 Human
Unigene: 337035 Mouse
Unigene: 138115 Rat
CXCR1主要表达于浸润的炎性细胞和肠上皮细胞表面.
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Sample:
Lane 1: Mouse Lymph node tissue lysates
Lane 2: Rat Lymph node cell lysates
Primary: Anti-CXCR1 (SL1009R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 40 kDa
Observed band size: 45 kDa
Sample:
Lane 1: Mouse Lung tissue lysates
Lane 2: Human Jurkat cell lysates
Lane 3: Human Raji cell lysates
Primary: Anti-CXCR1 (SL1009R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 40 kDa
Observed band size: 45 kDa
Tissue/cell: rat colitis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-CXCR1/IL-8RA Polyclonal Antibody, Unconjugated(SL1009R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
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