Rabbit Anti-KGF antibody | Sunlong Medical

Home > Product > Antibody > Rabbit Anti-KGF antibody

FGF 7; FGF7; FGF-7; Fibroblast Growth Factor 7;HBGF 7; HBGF7; HBGF-7; Keratinocyte growth factor precursor; Heparin binding growth factor 7; Keratinocyte Growth Factor; FGF7_HUMAN; Heparin-binding growth factor 7.

Cat:

SL0734R

Species Reactivity：

Human,Rat,(predicted: Mouse,Chicken,Dog,Pig,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human KGF:101-194/194

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KGF) Polyclonal Antibody, Unconjugated (SL0734R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-KGF/FGF7 Polyclonal Antibody, Unconjugated(SL0734R) 1:300, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

prev next

Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
Feedback Wall

KGF is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein is a potent epithelial cell specific growth factor, whose mitogenic activity is predominantly exhibited in keratinocytes but not in fibroblasts and endothelial cells. Studies of mouse and rat homologs of this gene implicated roles in morphogenesis of epithelium, reepithelialization of wounds, hair development and early lung organogenesis.

Function:
Plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation. Required for normal branching morphogenesis. Growth factor active on keratinocytes. Possible major paracrine effector of normal epithelial cell proliferation.

Subunit:
Interacts with FGFBP1. Interacts with FGFR2. Affinity between fibroblast growth factors (FGFs) and their receptors is increased by heparan sulfate glycosaminoglycans that function as coreceptors.

Subcellular Location:
Secreted.

Tissue Specificity:
Epithelial cell.

Similarity:
Belongs to the heparin-binding growth factors family.

SWISS:
P21781

Gene ID:
2252

Database links:

Entrez Gene: 2252 Human

Entrez Gene: 14178 Mouse

Entrez Gene: 29348 Rat

Omim: 14836 Human

SwissProt: P21781 Human

SwissProt: P36363 Mouse

SwissProt: Q02195 Rat

Unigene: 567268 Human

Unigene: 330557 Mouse

Unigene: 98842 Rat

KGF/FGF7 (Fibroblast growth factor 7) 又称：HBGF-7 纤维母细胞生长因子7，是FGF家族的主要成员之一。
FGF家族参与细胞生长、组织修复、胚胎生长发育、组织器官的形成、肿瘤的生长和侵润等诸多生理病理功能。
成纤维细胞生长因子7的促增生作用主要来源于角化上皮细胞而不是间质细胞和内皮细胞，因此人们又称之为：角化上皮细胞生长因子（KGF）。 Picture

Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-KGF/FGF7 Polyclonal Antibody, Unconjugated(SL0734R) 1:300, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Product Feedback Wall