Gelsolin is a calcium dependent actin binding protein. It is a potent modulator of actin filament length and gelation. Gelsolin has been shown to exist in at least two variant forms, cytoplasmic gelsolin and plasma gelsolin. Plasma gelsolin has also been called actin depolymerizing factor (ADF) or brevin. Human plasma and rabbit macrophage gelsolins differ by the presence of a 25 amino acid residue extension at the NH2 terminus in the human plasma gelsolin, which appears to account for the difference in relative molecular weights.
Function:
Calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Plays a role in ciliogenesis.
Subunit:
Binds to actin and to fibronectin. Identified in a complex composed of ACTA1, COBL, GSN AND TMSB4X.
Subcellular Location:
Isoform 2: Cytoplasm, cytoskeleton. Isoform 1: Secreted.
Tissue Specificity:
Phagocytic cells, platelets, fibroblasts, nonmuscle cells, smooth and skeletal muscle cells.
Post-translational modifications:
Phosphorylation on Tyr-86, Tyr-409, Tyr-465, Tyr-603 and Tyr-651 in vitro is induced in presence of phospholipids.
DISEASE:
Amyloidosis 5 (AMYL5) [MIM:105120]: A hereditary generalized amyloidosis due to gelsolin amyloid deposition. It is typically characterized by cranial neuropathy and lattice corneal dystrophy. Most patients have modest involvement of internal organs, but severe systemic disease can develop in some individuals causing peripheral polyneuropathy, amyloid cardiomyopathy, and nephrotic syndrome leading to renal failure. Note=The disease is caused by mutations affecting the gene represented in this entry.
Similarity:
Belongs to the villin/gelsolin family.
Contains 6 gelsolin-like repeats.
SWISS:
P06396
Gene ID:
2934
Database links:
Entrez Gene: 2934 Human
Entrez Gene: 227753 Mouse
Entrez Gene: 296654 Rat
Omim: 137350 Human
SwissProt: P06396 Human
SwissProt: P13020 Mouse
SwissProt: Q68FP1 Rat
Unigene: 522373 Human
Unigene: 21109 Mouse
Unigene: 103770 Rat
凝溶胶蛋白(gelsolin)是凝溶胶蛋白超家族的成员之一,是一种重要的肌动蛋白结合蛋白,其通过切断、封端肌动蛋白丝,或使肌动蛋白聚集成核等方式来控制肌动蛋白的结构. 还在细胞运动、控制细胞程序性死亡等细胞活动中发挥重要的作用.此外,凝溶胶蛋白在肿瘤细胞中的表达量也发生一定的变化. gelsolin也参与细胞调节作用。
| Picture |
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Gelsolin) Polyclonal Antibody, Unconjugated (SL1160R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Gelsolin) Polyclonal Antibody, Unconjugated (SL1160R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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