Rabbit Anti-IQGAP1 antibody

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IQ motif containing GTPase activating protein 1; KIAA0051; p195; Ras GTPase activating like protein 1; IQGA1_HUMAN.

Cat:

SL1109R

Species Reactivity：

Human,Rat,Rabbit,(predicted: Mouse,)

Immunogen：

KLH conjugated synthetic peptide derived from human IQGAP1:1451-1550/1657

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500Flow-Cyt=2ug/TestIF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Tissue/cell: rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-IQGAP1 Polyclonal Antibody, Unconjugated(SL1109R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingTissue/cell: rabbit placenta tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-IQGAP1 Polyclonal Antibody, Unconjugated(SL1109R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingBlank control: U87MG. Primary Antibody (green line): Rabbit Anti-IQGAP1 antibody (SL1109R) Dilution: 2ug/Test; Secondary Antibody : Goat anti-rabbit IgG-FITCDilution: 0.5ug/Test. ProtocolThe cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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IQGAP1 is a widely expressed protein than interacts with components of the cytoskeleton, with cell adhesion molecules, and with several signaling molecules to regulate cell morphology. It binds to activated CDC42 but does not stimulate its GTPase activity. It associates with calmodulin. Could serve as an assembly scaffold for the organization of a multimolecular complex that would interface incoming signals to the reorganization of the actin cytoskeleton at the plasma membrane. May promote neurite outgrowth.

Function:
Binds to activated CDC42 but does not stimulate its GTPase activity. It associates with calmodulin. Could serve as an assembly scaffold for the organization of a multimolecular complex that would interface incoming signals to the reorganization of the actin cytoskeleton at the plasma membrane. May promote neurite outgrowth.

Subunit:
Interacts with CDC42; the interaction is demonstrated with IQGAP1 in GTP-bound and in nucleotide-free state. Interacts with RAC1. Does not interact with RHOA. Interacts with TSG101. Interacts with PAK6.

Subcellular Location:
Cell membrane.

Tissue Specificity:
Expressed in the placenta, lung, and kidney. A lower level expression is seen in the heart, liver, skeletal muscle and pancreas.

Post-translational modifications:
Phosphorylation of Ser-1443 by PKC/PRKCE prevents interaction between C1 and C2, allowing binding of nucleotide-free CDC42. Ser-1443 phosphorylation enhances the ability to promote neurite outgrowth.

Similarity:
Contains 1 CH (calponin-homology) domain.
Contains 4 IQ domains.
Contains 1 Ras-GAP domain.
Contains 1 WW domain.

SWISS:
P46940

Gene ID:
8826

Database links:

Entrez Gene: 8826 Human

Entrez Gene: 29875 Mouse

Entrez Gene: 361598 Rat

Omim: 603379 Human

SwissProt: P46940 Human

SwissProt: Q9JKF1 Mouse

Unigene: 430551 Human

Unigene: 207619 Mouse

Unigene: 106939 Rat

支架蛋白IQGAP1与许多重要的细胞活动紧密相关，如黏附和迁移。研究发现在损伤修复、组织发展、肿瘤转移等过程中，IQGAP1的表达和功能有明显改变。同时作为Rh.GTPase信号的主要效应因子，IQGAP1能传递细胞外信号至黏附素复合体、细胞骨架以及细胞核。通过上述信号通路，IQ-GAP1协助细胞完成一系列重要生理病理活动。 Picture

Tissue/cell: rabbit placenta tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-IQGAP1 Polyclonal Antibody, Unconjugated(SL1109R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Blank control: U87MG.
Primary Antibody (green line): Rabbit Anti-IQGAP1 antibody (SL1109R)
Dilution: 2ug/Test;
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 0.5ug/Test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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