Rabbit Anti-MAdCAM-1 antibody

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Mucosal addressin cellular adhesion molecule-1; Addressin mucosal; hMAdCAM 1; MACAM1; Mucosal addressin cell adhesion molecule 1; Mucosal vascular addressin cell adhesion molecule 1; MADCA_RAT.

Cat:

SL0642R

Species Reactivity：

Human,Mouse,(predicted: Rat,)

Immunogen：

KLH conjugated synthetic peptide derived from rat MAdCAM-1:151-250/394

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Lymph node (Mouse) Lysate at 30 ugSpleen (Mouse) Lysate at 30 ugPrimary: Anti- MAdCAM-1 (SL0642R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 40 kDObserved band size: 50 kDTissue/cell: human gastric carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37∩ for 20 min; Incubation: Anti-MAdCAM-1 Polyclonal Antibody, Unconjugated(SL0642R) 1:200, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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The protein encoded by this gene is an endothelial cell adhesion molecule that interacts preferentially with the leukocyte beta7 integrin LPAM-1 (alpha4beta7), L-selectin, and VLA-4 (alpha4beta1) on myeloid cells to direct leukocytes into mucosal and inflamed tissues. It is a member of the immunoglobulin family and is similar to ICAM1 and VCAM1. At least seven alternatively spliced transcripts encoding different protein isoforms have been found for this gene, but the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008]

Function:
Cell adhesion leukocyte receptor expressed by mucosal venules, helps to direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria. It can bind both the integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes (By similarity).

Subunit:
Homodimer (Probable).

Subcellular Location:
Membrane; Single-pass type I membrane protein.

Tissue Specificity:
Detected in Peyer patches and mesenteric lymph nodes but not in spleen.

Similarity:
Contains 3 Ig-like (immunoglobulin-like) domains.

SWISS:
O70540

Gene ID:
54266

Database links:

Entrez Gene: 17123 Mouse

SwissProt: Q61826 Mouse

Unigene: 391556 Mouse

MAdCAM-1（粘膜血管定居因子或黏膜地址素细胞黏附分子）、CD44等均参与癌症和肿瘤细胞与血管内皮的相互作用，与癌症和肿瘤细胞一起穿过血管内皮而进入血管内，参与血行播散转移。 Picture

Tissue/cell: human gastric carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37∩ for 20 min;
Incubation: Anti-MAdCAM-1 Polyclonal Antibody, Unconjugated(SL0642R) 1:200, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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