Rabbit Anti-MIF antibody | Sunlong Medical

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Macrophage Migration Inhibitory Factor; GIF; GLIF; Glycosylation inhibiting factor; MIF protein; MMIF; Phenylpyruvate tautomerase; acrophage migration inhibitory factor (glycosylation-inhibiting factor); L-dopachrome isomerase; MIF_HUMAN.

Cat:

SL1044R

Species Reactivity：

Human,Rat,(predicted: Mouse,Dog,Pig,Cow,)

Immunogen：

KLH conjugated synthetic peptide derived from human MIPF:51-115/115

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Lane 1: Rat Aldrenal gland Lysates Lane 2: Human HL-60 cell Lysates Primary: Anti-MIF (SL1531R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 12kDaObserved band size: 12kDaTissue/cell: skin of rat foot tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-MIF Polyclonal Antibody, Unconjugated(SL1044R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingTissue/cell: human glioma tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-MIF Polyclonal Antibody, Unconjugated(SL1044R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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This gene encodes a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways. [provided by RefSeq, Jul 2008]

Function:
Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity.

Subunit:
Homotrimer. Interacts with CXCR2 extracellular domain. Interacts with the CD74 extracellular domain, COPS5 and BNIPL.

Subcellular Location:
Secreted. Cytoplasm. Note=Does not have a cleavable signal sequence and is secreted via a specialized, non-classical pathway. Secreted by macrophages upon stimulation by bacterial lipopolysaccharide (LPS), or by M.tuberculosis antigens.

DISEASE:
Genetic variations in MIF are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.

Similarity:
Belongs to the MIF family.

SWISS:
P14174

Gene ID:
4282

Database links:

Entrez Gene: 4282 Human

Entrez Gene: 17319 Mouse

Entrez Gene: 81683 Rat

Omim: 153620 Human

SwissProt: P14174 Human

SwissProt: P34884 Mouse

SwissProt: P30904 Rat

Unigene: 407995 Human

Unigene: 2326 Mouse

Unigene: 2661 Rat

Unigene: 2326 Mouse

Unigene: 2661 Rat

生长因子和激素（ Growth Factor and Hormones）
巨噬细胞移动抑制因子是一种前炎症因子,可由多种细胞产生,它既是一种细胞因子,又是一种源于垂体的激素,还可以作为糖皮质激素生理活动的负反馈调节剂.随着巨噬细胞移动抑制因子cDNA的成功克隆及其结构的阐明,巨噬细胞移动抑制因子的特殊生物学活性及其在多种疾病中的重要作用已日益为科研人员所重视。 Picture

Tissue/cell: skin of rat foot tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-MIF Polyclonal Antibody, Unconjugated(SL1044R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Tissue/cell: human glioma tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-MIF Polyclonal Antibody, Unconjugated(SL1044R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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