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Home > Product > Antibody > Rabbit Anti-Nociceptin antibody
PNOC; N/OFQ; N23K; N23K/N27K; Nociceptin, included; Npnc1; OFQ; OFQ/N; Orphanin FQ; PPNOC; prepronociceptin; Propronociceptin; PNOC_HUMAN.
Cat:
SL0075R
Species Reactivity：
Rat,(predicted: Human,Mouse,Dog,Pig,Cow,Horse,Rabbit,)
Immunogen：
KLH conjugated synthetic peptide derived from human Nociceptin:130-146/176
Format：
Liquid
Storage instructions：
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration：
1mg/ml
Clonality：
Polyclonal
Isotype：
IgG
Applications：
WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host：
Rabbit
Product Overview：
Sample: Brain(Rat) lysate at 30ug; Liver(Rat) lysate at 30ug; Primary: Anti-Nociceptin(SL0075R) at 1:200 dilution; Secondary: HRP conjugated Goat-Anti-Rabbit IgG(SL0295G-HRP) at 1: 3000 dilution; Predicted band size : 20kDObserved band size : 20kDTissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-Nociceptin Polyclonal Antibody, Unconjugated(SL0075R) 1:300, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
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Product PDFs
Datasheet:


Nociceptin (PNOC) is the ligand of the opioid receptor-like receptor (OPRL1). It may act as a transmitter in the brain by modulating nociceptive and locomotor behavior. May be involved in neuronal differentiation and development. Expressed predominantly in the spinal cord and brain, being more abundant in the hypothalamus and striatum. Also found in small amounts in ovary. Belongs to the opioid neuropeptide precursor family.


Function:
Nociceptin is the ligand of the opioid receptor-like receptor (OPRL1). It may act as a transmitter in the brain by modulating nociceptive and locomotor behavior. May be involved in neuronal differentiation and development (By similarity).

Subcellular Location:
Secreted.

Tissue Specificity:
Predominantly in the brain and spinal cord.

Similarity:
Belongs to the opioid neuropeptide precursor family.

SWISS:
Q13519

Gene ID:
5368

Database links:

Entrez Gene: 5368 Human

Entrez Gene: 18155 Mouse

Entrez Gene: 25516 Rat

Omim: 601459 Human

SwissProt: Q13519 Human

SwissProt: Q64387 Mouse

SwissProt: Q62923 Rat

Unigene: 88218 Human

Unigene: 16347 Mouse

Unigene: 486163 Mouse

Unigene: 87935 Rat



孤菲肽（Nociceptin又称为Orphanin FQ; PPNOC; ORL1）是新发现的一种神经肽，由17个氨基酸组成，又称痛敏肽。消化道肿瘤病人的血浆中OFQ含量明显高于对照组，尤以结肠癌、胃癌最为明显。 Picture

Sample:
Brain(Rat) lysate at 30ug;
Liver(Rat) lysate at 30ug;
Primary: Anti-Nociceptin(SL0075R) at 1:200 dilution;
Secondary: HRP conjugated Goat-Anti-Rabbit IgG(SL0295G-HRP) at 1: 3000 dilution;
Predicted band size : 20kD
Observed band size : 20kD
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-Nociceptin Polyclonal Antibody, Unconjugated(SL0075R) 1:300, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
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