Rabbit Anti-Osteopontin antibody

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BNSP; Bone sialoprotein 1; BSP I; BSPI; Early T lymphocyte activation 1; ETA 1; ETA1; MGC110940; Nephropontin; OPN; osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein; OSTP_HUMAN; secreted phosphoprotein 1 (osteopontin, bone sia

Cat:

SL0019R

Species Reactivity：

Human,(predicted: Pig,Cow,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human OPN:141-220/314

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: A549 Cell Lysate at 40 ugPrimary: Anti-ostepontin (SL0019R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 35 kDObserved band size: 50 kD

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Osteopontin is the principal phosphorylated glycoprotein of bone and is expressed in a limited number of other tissues including dentine. Osteopontin is produced by osteoblasts under stimulation by calcitriol and binds tightly to hydroxyapatite. It is also involved in the anchoring of osteoclasts to the mineral of bone matrix via the vitronectin receptor, which has specificity for osteopontin. Osteopontin is overexpressed in a variety of cancers, including lung, breast, colorectal, stomach, ovarian, melanoma and mesothelioma.

Function:
Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.
Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity (By similarity).

Subunit:
Ligand for integrin alpha-V/beta-3.

Subcellular Location:
Secreted.

Tissue Specificity:
Bone. Found in plasma.

Similarity:
Belongs to the osteopontin family.

SWISS:
P10451

Gene ID:
6696

Database links:

Entrez Gene: 6696 Human

Entrez Gene: 20750 Mouse

Entrez Gene: 25353 Rat

Omim: 166490 Human

SwissProt: P10451 Human

SwissProt: P10923 Mouse

SwissProt: P08721 Rat

Unigene: 313 Human

Unigene: 288474 Mouse

Unigene: 8871 Rat

信号传导（Signaling Intermediates）
骨桥蛋白(osteopontin，OPN)是一种含精氨酸—甘氨酸—天冬氨酸(Arg-Gly-Asp，RGD)的分泌型糖基化磷蛋白，已归类于细胞外基质(extracellular matrix，ECM)蛋白。 OPN既是细胞因子，又是细胞黏附分子.又称：分泌性磷酸化蛋白质1
OPN最初是从骨组织分离的，而后在上皮细胞、胃肠道的分泌物、肾、甲状腺、睾丸以及一些肿瘤细胞里都陆续的发现了OPN的表达。在生殖方面，OPN在分泌期子宫内膜上皮细胞、侵袭的滋养细胞、蜕膜细胞中表达。 Picture

Sample: A549 Cell Lysate at 40 ug
Primary: Anti-ostepontin (SL0019R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 35 kD
Observed band size: 50 kD

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