Rabbit Anti-PACAP receptor antibody

Home > Product > Antibody > Rabbit Anti-PACAP receptor antibody

ADCYAP1 R1; ADCYAP1R 1; PACAP-R1; adenylate cyclase activating polypeptide 1 (pituitary) receptor type 1; PAC 1; PAC1; PAC1R; PACAP R 1; PACAP1 R; PACAPR; PACAPRI; Pituitary adenylate cyclase activating polypeptide 1 receptor type I Hiphop; Pituitary aden

Cat:

SL0198R

Species Reactivity：

Mouse,Rat,(predicted: Human,Chicken,Dog,Horse,Rabbit,)

Immunogen：

KLH conjugated synthetic peptide derived from the middle of human PACAP R1:251-330/468

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample:Cerebrum (Mouse) Lysate at 40 ugPrimary: Anti-PACAP receptor(SL0198R)at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 51kDObserved band size: 48kDTissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-PACAP R1/PACAP receptor 1 Polyclonal Antibody, Unconjugated(SL0198R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

prev next

Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
Feedback Wall

Pituitary adenylate cyclase-activating polypeptide (PACAP) is a neuropeptide belonging to the vasoactive intestinal polypeptide/glucagon/ secretin family. It is widely distributed in the body, and a variety of biological actions have been reported. Recent studies have shown that there is a family of PACAP receptors (PACAPRs), and two members of this family have been identified. This is a receptor for PACAP-27 and PACAP-38. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. May regulate the release of adrenocorticotropin, luteinizing hormone, growth hormone, prolactin, epinephrine, and catecholamine. May play a role in spermatogenesis and sperm motility. Causes smooth muscle relaxation and secretion in the gastrointestinal tract (By similarity). Belongs to the G-protein coupled receptor 2 family.

Function:
This is a receptor for PACAP-27 and PACAP-38. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. May regulate the release of adrenocorticotropin, luteinizing hormone, growth hormone, prolactin, epinephrine, and catecholamine. May play a role in spermatogenesis and sperm motility. Causes smooth muscle relaxation and secretion in the gastrointestinal tract.

Subunit:
Interacts (via N-terminal extracellular domain) with ADCYAP1.

Subcellular Location:
Cell membrane; Multi-pass membrane protein.

Tissue Specificity:
Most abundant in the brain, low expression in the lung, liver, thymus, spleen, pancreas and placenta.

Similarity:
Belongs to the G-protein coupled receptor 2 family.

SWISS:
P41586

Gene ID:
117

Database links:

Entrez Gene: 117 Human

Entrez Gene: 319095 Cow

Entrez Gene: 11517 Mouse

Entrez Gene: 24167 Rat

Omim: 102981 Human

SwissProt: Q29627 Cow

SwissProt: P41586 Human

SwissProt: P70205 Mouse

SwissProt: P32215 Rat

Unigene: 377783 Human

Unigene: 44245 Mouse

Unigene: 81688 Rat

生长因子和激素（ Growth Factor and Hormones）
PACAP 受体分为两个亚型，PACAP-1型受体对PACAP是特异性的，与PACAP的亲和力比VIP高100-1000倍，PACAP-II型受体与PACAP和VIP的亲和力相似，对二者无选择性。此抗体即为前者。 Picture

Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-PACAP R1/PACAP receptor 1 Polyclonal Antibody, Unconjugated(SL0198R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Product Feedback Wall