cell death activator CIDE-B; Cell death activator CIDE B; Cell death inducing DFFA like effector B; CIDEB_HUMAN; cell death-inducing DFFA-like effector B; Cide-b.
Cat:
SL0716R
Species Reactivity：
Human,Mouse,Rat,(predicted: Dog,Pig,Cow,Horse,)
Immunogen：
KLH conjugated synthetic peptide derived from human CIDEB:118-219/219
Format：
Liquid
Storage instructions：
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration：
1mg/ml
Clonality：
Polyclonal
Isotype：
IgG
Applications：
WB=1:500-2000ELISA=1:5000-10000IF=1:100-500not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host：
Rabbit
Product Overview：
Sample: Lane 1: Rat Small intestine tissue lysatesLane 2: Human HL-60 cell lysates Primary: Anti-CIDEB (SL0716R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 24 kDaObserved band size: 27 kDaSample: Large intestine (Mouse) Lysate at 40 ugRaw264.7(Mouse) Cell Lysate at 30 ugPrimary: Anti- CIDEB (SL0716R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 24 kDObserved band size: 27 kDTissue/cell: mouse intestine tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-CIDEB Polyclonal Antibody, Unconjugated(SL0716R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. DFF45/ICAD has been identified as an inhibitor of caspase activated DNase DFF40/CAD. DFF45 related proteins CIDE A and CIDE B were recently identified. CIDE contains a new type of domain termed CIDE N, which has high homology with the regulatory domains of DFF45/ICAD and DFF40/CAD. Expression of CIDE B induces apoptosis, which is inhibited by DFF45. CIDE B is a DFF45 inhibitable effector that promotes cell death and DNA fragmentation. CIDE B is expressed mainly in liver and small intestine and at lower levels in spleen, colon, kidney, peripheral blood lymphocytes, and bone marrow.
Function: Activates apoptosis.
Subunit: Inhibited by DFFB. Interacts with DFFA and DFFB.
Tissue Specificity: Highly expressed in liver and small intestine and, at lower levels, in colon, kidney and spleen.
Sample:
Lane 1: Rat Small intestine tissue lysates
Lane 2: Human HL-60 cell lysates
Primary: Anti-CIDEB (SL0716R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 24 kDa
Observed band size: 27 kDa
Sample:
Large intestine (Mouse) Lysate at 40 ug
Raw264.7(Mouse) Cell Lysate at 30 ug
Primary: Anti- CIDEB (SL0716R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 24 kD
Observed band size: 27 kD
Tissue/cell: mouse intestine tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-CIDEB Polyclonal Antibody, Unconjugated(SL0716R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
