Rabbit Anti-Myc tag antibody

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avian myelocytomatosis viral oncogene homolog; bHLHe39; c-Myc; C-Myc-Tag; class E basic helix-loop-helix protein 39; MRTL; MYC; Myc Epitope Tag; myc proto-oncogene protein; myc-related translation/localization regulatory factor; oncogene c-Myc;

Cat:

SL0842R

Species Reactivity：

Recombinant protein

Immunogen：

KLH conjugated C-EQKLISEEDL (human Proto-oncogene c-Myc)

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:1000-5000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Lane1: Myc-Tagged Fusion Protein Overexpression E.coli Lysate at 2ugLane2: Myc-Tagged Fusion Protein Overexpression E.coli Lysate at 2ugPrimary: Anti-Myc tag (SL0842R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 41 kDObserved band size: 45 kDTissue/cell: human endometrium tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-SLCMyc Polyclonal Antibody, Unconjugated(SL0842R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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Unit:

Price: $136.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties. The Myc epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast,insect and mammalian cell systems.

The c-Myc protein is a transcription factor, which is encoded by the c-Myc gene on human chromosome 8q24. c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. The phosphorylation of c-Myc has been investigated and previous studies have suggested a functional association between phosphorylation at Thr58/Ser62 by glycogen synthase kinase 3, cyclin dependent kinase, ERK2 and SLCJun N terminal Kinase (JNK) in cell proliferation and cell cycle regulation. Studies also have shown that c-Myc is essential for tumor cell development in vasculogenesis and angiogenesis that distribute blood throughout the cells, and which brought extensive attention in the development of new therapeutic approach for cancer treatment.

Subunit:
Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a heterodimer with MAX. Interacts with TAF1C and SPAG9. Interacts with PARP10. Interacts with KDM5A and KDM5B. Interacts (when phosphorylated at Thr-58 and Ser-62) with FBXW7. Interacts with PIM2 (By similarity). Interacts with NO66.

Subcellular Location:
Nucleus, nucleoplasm. Nucleus, nucleolus.

Similarity:
Contains 1 basic helix-loop-helix (bHLH) domain.

SWISS:
P01106

Gene ID:
929

Database links:

Entrez Gene: 929 Human

Entrez Gene: 17869 Mouse

Entrez Gene: 24577 Rat

Omim: 190080 Human

SwissProt: P01106 Human

SwissProt: P01108 Mouse

SwissProt: P09416 Rat

Unigene: 202453 Human

Unigene: 2444 Mouse

Unigene: 12072 Rat

Picture

Tissue/cell: human endometrium tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-SLCMyc Polyclonal Antibody, Unconjugated(SL0842R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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