The protein encoded by this gene is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. This gene is thought to encode a secreted protein which negatively regulates skeletal muscle growth. Acts specifically as a negative regulator of skeletal muscle growth. [SUBUNIT] Homodimer; [TISSUE SPECIFICITY] Expressed specifically in developing and adult skeletal muscle. Weak expression in adipose tissue. Belongs to the TGF-beta family.
Function:
Acts specifically as a negative regulator of skeletal muscle growth.
Subunit:
Homodimer; disulfide-linked. Interacts with WFIKKN2, leading to inhibit its activity. Interacts with FST3.
Subcellular Location:
Secreted
Tissue Specificity:
Predominantly expressed in muscle. At hatching, expression is strongest in the skin epithelium, and is also found in the retina and brain. From day 28, expressed in skeletal muscle. In the adult, highest expression is seen in the gastrointestinal tract, brain, muscle, heart and testis. Also expressed in the adult pharynx, kidney, spleen, liver, gill, eyes, skin, swim bladder and ovary.
DISEASE:
Defects in MSTN are the cause of muscle hypertrophy (MSLHP) [MIM:614160]. MSLHP is a condition characterized by increased muscle bulk and strength. Affected individuals are exceptionally strong.
Similarity:
Belongs to the TGF-beta family.
SWISS:
O14793
Gene ID:
2660
Database links:
Entrez Gene: 2660 Human
Entrez Gene: 17700 Mouse
Entrez Gene: 373964 Chicken
Entrez Gene: 399534 Pig
Entrez Gene: 29152 Rat
Entrez Gene: 798441 Zebrafish
Omim: 601788 Human
SwissProt: O14793 Human
SwissProt: O08689 Mouse
SwissProt: O35312 Rat
SwissProt: O18830 Sheep
SwissProt: O42222 Zebrafish
Unigene: 41565 Human
Unigene: 3514 Mouse
Unigene: 4492 Rat
GDF-8又称MSTN,是转化生长因子超家族,也是近年来发现的一类重要的肌细胞生长调控因子,它通过抑制MyoD家族成员转录活性负向控制肌细胞的生长发育,它的表达量与肌肉重量的变化呈负相关。
Picture |
Sample:
Lane 1: Muscle (Mouse) Lysate at 40 ug
Lane 2: Muscle (Rat) Lysate at 40 ug
Lane 3: Tongue (Rat) Lysate at 40 ug
Primary:
Anti-GDF8 (SL1288R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 43 kD
Observed band size: 43 kD
Paraformaldehyde-fixed, paraffin embedded (mouse skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Anti-GDF8 ) Polyclonal Antibody, Unconjugated (SL1288R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat tongue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Anti-GDF8 ) Polyclonal Antibody, Unconjugated (SL1288R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: mouse intestine tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-GDF8/MSTN Polyclonal Antibody, Unconjugated(SL1288R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
|
|
|