Rabbit Anti-Parvalbumin antibody

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D22S749; MGC116759; Parvalbumin alpha; PRVA_HUMAN; PVALB.

Cat:

SL1299R

Species Reactivity：

Human,Mouse,Rat,(predicted: Chicken,Pig,Cow,Horse,)

Immunogen：

KLH conjugated synthetic peptide derived from human Parvalbumin:7-110/110

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500Flow-Cyt=1ug/TestIF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Lane 1: Mouse Kidney tissue lysates Lane 2: Rat Kidney tissue lysates Primary: Anti-Parvalbumin (SL1299R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 12 kDaObserved band size: 10 kDaParaformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (Parvalbumin) Polyclonal Antibody, Unconjugated (SL1299R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (Parvalbumin) Polyclonal Antibody, Unconjugated (SL1299R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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The protein encoded by this gene is a high affinity calcium ion-binding protein that is structurally and functionally similar to calmodulin and troponin C. The encoded protein is thought to be involved in muscle relaxation. Belongs to the parvalbumin family. Contains 2 EF-hand domains. This protein binds two calcium ions.

Function:
In muscle, parvalbumin is thought to be involved in relaxation after contraction. It binds two calcium ions.

Similarity:
Belongs to the parvalbumin family.
Contains 2 EF-hand domains.

SWISS:
P20472

Gene ID:
5816

Database links:

Entrez Gene: 396459 Chicken

Entrez Gene: 396531 Chicken

Entrez Gene: 5816 Human

Entrez Gene: 19293 Mouse

Entrez Gene: 25269 Rat

Omim: 168890 Human

SwissProt: P20472 Human

SwissProt: P32848 Mouse

SwissProt: P02625 Rat

Unigene: 295449 Human

Unigene: 2766 Mouse

Unigene: 2005 Rat

(Parvalbumin, PV)细小白蛋白属于钙结合蛋白家族,是一种细胞内可溶的小结合蛋白，过去曾认为，仅出现在哺乳动物某些肌纤维内,它与Ca2+有高度亲合力,与钙调蛋白竞争自由Ca2+,故可阻止肌浆内的Ca2+增高，可抗衡细胞内钙过量的发生。 Picture

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (Parvalbumin) Polyclonal Antibody, Unconjugated (SL1299R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (Parvalbumin) Polyclonal Antibody, Unconjugated (SL1299R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.

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