Rabbit Anti-TIE1 antibody | Sunlong Medical

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TIE1_HUMAN; JKT 14; JTK14; TIE; Tyrosine Kinase with Immunoglobulin and Epidermal Growth Factor Homology Domains; Tyrosine protein kinase receptor Tie 1; Tyrosine-protein kinase receptor Tie-1; tyrosine kinase receptor 1; tyrosine kinase with immunoglobul

Cat:

SL1334R

Species Reactivity：

Human,(predicted: Mouse,Rat,)

Immunogen：

KLH conjugated synthetic peptide derived from human Tie1:851-1000/1138

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: A549(Human) Cell Lysate at 30 ugHepG2(Human) Cell Lysate at 30 ugPrimary: Anti-TIE1 (SL1334R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 123 kDObserved band size: 123 kDTissue/cell: human brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-TIE1 Polyclonal Antibody, Unconjugated(SL1334R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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TIE1/TIE (tyrosine kinase with Ig and EGF homology domains 1) and TIE2/Tek define a new class of the receptor tyrosine kinase (RTK) subfamily with unique structural characteristics: two immunoglobulin like domains flanking three epidermal growth factor (EFG) like domains followed by three fibronectin type III like repeats in the extracellular region and a split tyrosine kinase domain in the cytoplasmic region. Human TIE1 cDNA encodes a 228 amino acid residue precursor protein with a putative signal peptide, an extracellular domain, and a cytoplasmic domain. Human TIE1/Fc, a disulfide linked homodimeric protein, has a calculated molecular mass of approximately 107 kDa. Due to glycosylation, the protein migrates to approximately 160 kDa in SDS PAGE under reducing conditions. TIE1 and TIE2, expressed primarily on endothelial and hematopoietic progenitor cells, play important roles in angiogenesis, vasculogenesis, and hematopoiesis. In developing vascular endothelial cells, TIE1 and TIE2 are specifically expressed. Two ligands that bind TIE have been identified, angiopoietin 1 and angiopoietin 2. Based on gene targeting studies, the in vivo functions of TIE1 are related to endothelial cell differentiation. The receptor tyrosine kinase TIE also plays a role in the survival and integrity of the endothelium.

Function:
Transmembrane tyrosine-protein kinase that may modulate TEK/TIE2 activity and contribute to the regulation of angiogenesis.

Subunit:
Heterodimer with TEK/TIE2.

Subcellular Location:
Cell membrane; Single-pass type I membrane protein.

Tissue Specificity:
Specifically expressed in developing vascular endothelial cells.

Post-translational modifications:
Phosphorylated on tyrosine residues in response to ANGPT1, most likely by TEK/TIE2.

Similarity:
Belongs to the protein kinase superfamily. Tyr protein kinase family. Tie subfamily.
Contains 3 EGF-like domains.
Contains 3 fibronectin type-III domains.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain.

SWISS:
P35590

Gene ID:
7075

Database links:

Entrez Gene: 280941 Cow

Entrez Gene: 7075 Human

Entrez Gene: 21846 Mouse

Entrez Gene: 81966 Rat

Omim: 600222 Human

SwissProt: Q01365 Cow

SwissProt: P35590 Human

SwissProt: Q01366 Mouse

Unigene: 78824 Human

Unigene: 4345 Mouse

Unigene: 13171 Rat

血管生成素-1受体又称上皮生长因子样域酪氨酸激酶1或内皮酪氨酸激酶受体,Tie1在血管内皮细胞、腺上皮及间质细胞中均有表达，主要定位于细胞质。 Picture

Tissue/cell: human brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-TIE1 Polyclonal Antibody, Unconjugated(SL1334R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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