Rabbit Anti-NKG2D antibody | Sunlong Medical

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NKG2-D type II integral membrane protein; KLRK1; natural killer cell group 2D; NKG2-D isoform b; Klrk1; Nkrp2; NKG2-D-activating NK receptor; NK cell receptor D; Killer cell lectin-like receptor subfamily K; NK lectin-like receptor; CD314; CD 314; CD314 a

Cat:

SL0938R

Species Reactivity：

Human,Mouse,(predicted: Rat,)

Immunogen：

KLH conjugated synthetic peptide derived from rat NKG2D:121-219/219<Extracellular>

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500Flow-Cyt=2μg/TestIF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Lung (Mouse) Lysate at 40 ugPrimary: Anti- NKG2D (SL0938R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 25 kDObserved band size: 26 kDBlank control (blue line): Mouse thymus cells (blue). Primary Antibody (green line): Rabbit Anti-NKG2D antibody (SL0938R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-PEDilution: 1μg /test. ProtocolThe cells were fixed with 70% methanol (Overnight at 4℃) . Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.Blank control:SW96. Primary Antibody (green line): Rabbit Anti-NKG2D antibody (SL0938R) Dilution: 2ug/Test; Secondary Antibody : Goat anti-rabbit IgG-FITCDilution: 0.5ug/Test. ProtocolThe cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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This locus represents naturally occurring read-through transcription between the neighboring KLRC4 (killer cell lectin-like receptor subfamily C, member 4) and KLRK1 (killer cell lectin-like receptor subfamily K, member 1) genes on chromosome 12. The read-through transcript includes an alternate 5' exon and lacks a significant portion of the KLRC4 coding sequence, including the start codon, and it thus encodes the KLRK1 protein. [provided by RefSeq, Dec 2010]

Function:
Receptor for MICA, MICB, ULBP1, ULBP2, ULBP3 (ULBP2>ULBP1>ULBP3) and ULBP4. Plays a role as a receptor for the recognition of MHC class I HLA-E molecules by NK cells and some cytotoxic T-cells. Involved in the immune surveillance exerted by T- and B-lymphocytes.

Subunit:
Homodimer.

Subcellular Location:
Membrane; Single-pass type II membrane protein.

Tissue Specificity:
Natural killer cells. Expressed on essentially all CD56+CD3- NK cells from freshly isolated PBMC. Also detected in gamma-delta cells and CD8+ alpha-beta T-cells. Expressed in interferon-producing killer dendritic cells (IKDCs).

Similarity:
Contains 1 SLCtype lectin domain.

SWISS:
O70215

Gene ID:
24934

Database links:

Entrez Gene: 100528032 Human

Entrez Gene: 22914 Human

Entrez Gene: 27007 Mouse

Entrez Gene: 24934 Rat

Omim: 611817 Human

SwissProt: P26718 Human

SwissProt: O54709 Mouse

SwissProt: O70215 Rat

Unigene: 387787 Human

Unigene: 8217 Mouse

Unigene: 14544 Rat

NKG2-D出现在免疫细胞上表达的受体，NKG2D属C型凝集素家族跨膜蛋白，广泛表达在NK细胞、CD8+的αβT细胞和γδT细胞表面，可以提高NK细胞对肿瘤细胞的杀伤活性。 Picture

Blank control (blue line): Mouse thymus cells (blue).
Primary Antibody (green line): Rabbit Anti-NKG2D antibody (SL0938R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 70% methanol (Overnight at 4℃) . Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

Blank control:SW96.
Primary Antibody (green line): Rabbit Anti-NKG2D antibody (SL0938R)
Dilution: 2ug/Test;
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 0.5ug/Test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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Specific References (9) | SL0938R has been referenced in 9 publications.

[IF=6.166] Young Min Chung. et al. FOXO3-dependent suppression of PD-L1 promotes anticancer immune responses via activation of natural killer cells. Am J Cancer Res. 2022; 12(3): 1241–1263 IF,WB ; Mouse.

PubMed:35411241

[IF=13.751] Young Min Chung. et al. Sensitizing tumors to anti-PD-1 therapy by promoting NK and CD8+ T cells via pharmacological activation of FOXO3. J Immunother Cancer. 2021 Dec;9(12):e002772 IF ; Mouse.

PubMed:34887262

[IF=1.664] Song N et al. Synergistic anti-tumor effects of dasatinib and dendritic cell vaccine onmetastatic breast cancer in a mouse model. Oncology Letters.2018. IHSLCP ; Mouse.

PubMed:10.3892/ol.2018.8188

[IF=3.558] Meng H et al. ZG16 regulates PD-L1 expression and promotes local immunity in colon cancerTransl Oncol.2021 Feb;14(2):101003. IHC ; Human.

PubMed:3372168

[IF=2.38] Mozer-Lisewska, Iwona, et al. "Detection and Significance of Cytotoxic Cell Subsets in Biopsies of HCSLVInfected Human Livers." Archivum immunologiae et therapiae experimentalis (2013): 1-8. Human.

PubMed:24232187

[IF=3.202] Long Y et al. Downregulation of MCT 4 for lactate exchange promotes the cytotoxicity of NK cells in breast carcinoma.Cancer Med. 2018 Sep;7(9):4690-4700. IHSLCP ; Mouse.

PubMed:30051648

[IF=1.664] Song et al. Synergistic anti-tumor effects of dasatinib and dendritic cell vaccine on metastatic breast cancer in a mouse model. (2018) Oncol.Lett. 15:6831-6838 IHC ; mouse.

PubMed:29731862

[IF=1.73] Onishi,Hideya, et al. "Random Migration Contributes to Cytotoxicity of Activated CD8+ T-Lymphocytes But Not NK cells." Anticancer Research 34.8 (2014): 3947-3956. Mouse.

PubMed:25075016

[IF=3.73] Ruck, Tobias, et al. "CD4+ NKG2D+ T Cells Exhibit Enhanced Migratory and Encephalitogenic Properties in Neuroinflammation." PLOS ONE 8.11 (2013): e81455. IHSLCP ; Human.

PubMed:24282598