Rabbit Anti-PHLDA1 antibody

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Apoptosis associated nuclear protein; Apoptosis-associated nuclear protein; DT1P1B11; MGC131738; PHLA1_HUMAN; PHLDA1; PHRIP; Pleckstrin homology like domain family A member 1; Pleckstrin homology-like domain family A member 1; PQ rich protein; PQ-rich pro

Cat:

SL1364R

Species Reactivity：

Mouse,Rat,(predicted: Human,Dog,Cow,)

Immunogen：

KLH conjugated synthetic peptide derived from human PHLDA1:251-360/401

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PHLDA1) Polyclonal Antibody, Unconjugated (SL1364R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Tissue/cell: mouse pancreas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-PHLDA1/TDAG51 Polyclonal Antibody, Unconjugated(SL1364R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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PHLDA1 is involved in regulation of apoptosis induced by signaling of T cell receptor in murine lympocytes. As any potent regulator with pleiotropic effects related to its overexpression, PHLDA attracts attention in both cell biology research and human tissue pathology.

Function:
Seems to be involved in regulation of apoptosis. May be involved in detachment-mediated programmed cell death. May mediate apoptosis during neuronal development. May be involved in regulation of anti-apoptotic effects of IGF1. May be involved in translational regulation.

Subunit:
Interacts with RPL14, EIF3S7 and PABPC4.

Subcellular Location:
Cytoplasm. Cytoplasmic vesicle membrane. Nucleus, nucleolus. Note=Colocalizes with intracellular vesicles.

Tissue Specificity:
Widely expressed with highest levels in pancreas. Strongly expressed by benign melanocytic nevi, and progressively reduced expressed in primary and metastatic melanomas (at protein level).

Similarity:
Contains 1 PH domain.

SWISS:
Q8WV24

Gene ID:
22822

Database links:

Entrez Gene: 22822 Human

Omim: 605335 Human

SwissProt: Q8WV24 Human

Unigene: 602085 Human

TDAG51参与活化诱导的细胞凋亡，具有促进活化T细胞凋亡的作用,在免疫自稳中发挥重要作用。TDAG51基因在体内各组织中或多或少都有一定的表达，但其功能目前还不清楚，有待于进一步研究。 Picture

Tissue/cell: mouse pancreas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-PHLDA1/TDAG51 Polyclonal Antibody, Unconjugated(SL1364R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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