Rabbit Anti-BOB1 antibody | Sunlong Medical

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B-cell oct-binding protein 1; B-cell-specific coactivator OBF-1; POU domain class 2-associating factor 1; POU2AF1; OCT-binding factor 1; BOB-1; OCA-B. OBF1; OCA-B; OBF1_HUMAN; BOB.1.

Cat:

SL1418R

Species Reactivity：

Rat,(predicted: Human,Mouse,Chicken,Dog,Cow,Horse,)

Immunogen：

KLH conjugated synthetic peptide derived from human BOb-1:21-130/256

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-BOb-1/POU2AF1 Polyclonal Antibody, Unconjugated(SL1418R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingTissue/cell: rat spleen tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-BOb-1/POU2AF1 Polyclonal Antibody, Unconjugated(SL1418R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Enables transcription coactivator activity. Involved in positive regulation of transcription by RNA polymerase II. Part of RNA polymerase II transcription regulator complex. [provided by Alliance of Genome Resources, Nov 2021]

Function:
Transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers.

Subcellular Location:
Nucleus.

Tissue Specificity:
B-cell specific.

Post-translational modifications:
Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation (Probable).

DISEASE:
Note=A chromosomal aberration involving POU2AF1/OSF1 may be a cause of a form of B-cell leukemia. Translocation t(3;11)(q27;q23) with BCL6.

Similarity:
Belongs to the POU2AF1 family.

SWISS:
Q16633

Gene ID:
5450

Database links:

Entrez Gene: 5450 Human

Entrez Gene: 18985 Mouse

Omim: 601206 Human

SwissProt: Q16633 Human

SwissProt: Q64693 Mouse

Unigene: 654525 Human

Unigene: 897 Mouse

Picture

Tissue/cell: rat spleen tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-BOb-1/POU2AF1 Polyclonal Antibody, Unconjugated(SL1418R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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