Rabbit Anti-CD68 antibody | Sunlong Medical

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CD 68; CD68 antigen; CD68 molecule; DKFZp686M18236; GP110; Macrophage antigen CD68 (microsialin); macrosialin; SCARD1; Scavenger receptor class D member 1; macrosialin isoform A precursor; CD68_HUMAN; Macrosialin; Gp110; scavenger receptor class D, member

Cat:

SL1432R

Species Reactivity：

Human,Mouse,(predicted: Cow,)

Immunogen：

KLH conjugated synthetic peptide derived from human CD68:1-130/354<Extracellular>

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Lane 1: Raw264.7 (Mouse) Cell Lysate at 30 ug Lane 2: Lymph node (Rat) Cell Lysate at 30 ug Primary: Anti-CD68 (SL1432R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 110/185-200 kD Observed band size: 110 kDSample: Lane 1: A431 (Human) Cell Lysate at 30 ugLane 2: A549 (Human) Cell Lysate at 30 ugLane 3: Jurkat (Human) Cell Lysate at 30 ugLane 4: Huvec (Human) Cell Lysate at 30 ugPrimary: Anti-CD68 (SL1432R) at 1/500 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 110/185-200 kDObserved band size: 110 kDTissue/cell: human mammary cancer; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-CD68 Polyclonal Antibody, Unconjugated(SL1432R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingThis image was generously provided by Amanda Brown, Ph.D. from Johns Hopkins University School of Medicine. Paraffin embedded human brain tissue labeled with Rabbit Anti-CD68 Polyclonal Antibody, Unconjugated (SL1432R) at 1:50 followed by conjugation to the secondary antibody and Permanent Fast Red Quanto staining

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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This gene encodes a 110-kD transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It is a member of the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family. The protein primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. It is a type I integral membrane protein with a heavily glycosylated extracellular domain and binds to tissue- and organ-specific lectins or selectins. The protein is also a member of the scavenger receptor family. Scavenger receptors typically function to clear cellular debris, promote phagocytosis, and mediate the recruitment and activation of macrophages. Alternative splicing results in multiple transcripts encoding different isoforms. [provided by RefSeq, Jul 2008]

Function:
Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.

Subcellular Location:
Isoform Short: Cell membrane; Single-pass type I membrane protein. Isoform Long: Endosome membrane; Single-pass type I membrane protein. Lysosome membrane; Single-pass type I membrane protein.

Tissue Specificity:
Highly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites.

Post-translational modifications:
N- and O-glycosylated

Similarity:
Belongs to the LAMP family.

SWISS:
P34810

Gene ID:
968

Database links:

Entrez Gene: 968 Human

Entrez Gene: 12514 Mouse

Omim: 153634 Human

SwissProt: P34810 Human

SwissProt: P31996 Mouse

Unigene: 647419 Human

Unigene: 15819 Mouse

巨噬细胞标志物
CD68为巨噬细胞抗原又称酸性溶霉菌糖蛋白，表达在单核、巨嗜、粒细胞和一些梭形细胞呈阳性表达。 Picture

Sample:
Lane 1: A431 (Human) Cell Lysate at 30 ug
Lane 2: A549 (Human) Cell Lysate at 30 ug
Lane 3: Jurkat (Human) Cell Lysate at 30 ug
Lane 4: Huvec (Human) Cell Lysate at 30 ug
Primary: Anti-CD68 (SL1432R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 110/185-200 kD
Observed band size: 110 kD

Tissue/cell: human mammary cancer; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-CD68 Polyclonal Antibody, Unconjugated(SL1432R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

This image was generously provided by Amanda Brown, Ph.D. from Johns Hopkins University School of Medicine. Paraffin embedded human brain tissue labeled with Rabbit Anti-CD68 Polyclonal Antibody, Unconjugated (SL1432R) at 1:50 followed by conjugation to the secondary antibody and Permanent Fast Red Quanto staining

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