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Home > Product > Antibody > Rabbit Anti-CXCL10/IP10 antibody
10 kDa interferon gamma induced protein; C7; Chemokine (C X C motif) ligand 10; Chemokine CXC motif ligand 10; Crg 2; CRG2; CXCL 10; CXCL10; Gamma IP10; gIP 10; GIP10; IFI 10; IFI10; INP 10; INP10; Interferon activated gene 10; Interferon gamma induced ce
Cat:
SL1502R
Species Reactivity：
Human,Mouse,Rat,
Immunogen：
KLH conjugated synthetic peptide derived from mouse CXCL10:35-98/98
Format：
Liquid
Storage instructions：
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration：
1mg/ml
Clonality：
Polyclonal
Isotype：
IgG
Applications：
ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500Flow-Cyt=1ug/TestIF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host：
Rabbit
Product Overview：
Tissue/cell: rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-CXCL10 Polyclonal Antibody, Unconjugated(SL1502R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingC57BL/6 mice skin were fixed in pre-chilled MeOH and incubated at -20℃ for 10 minutes. They were washed in PBS at RT 3 times for 5 minutes each. The sections were blocked for 60 minutes at RT with PBS containing 5% BSA. The block was removed, anti-CXCL10 antibody (SL1502R) diluted 1:50 was added, then incubated overnight at 4℃.Then washed with PBS (0.005% Tween20) for 15 minutes each followed by 2 washes of PBS for 5 minutes each. The secondary antibody, anti-rabbit A488 was diluted 1:500, added to the sections and incubated for 1 hour at RT. Then washed for 10 minutes in PBS 4 timesBlank control: Raw264.7. Primary Antibody (green line): Rabbit Anti-CXCL10/IP10 antibody (SL1502R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647Dilution: 1μg /test. ProtocolThe cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.Blank control: Raw264.7. Primary Antibody (green line): Rabbit Anti-CXCL10/IP10 antibody (SL1502R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647Dilution: 1μg /test. ProtocolThe cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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Product PDFs
Datasheet:


This antimicrobial gene encodes a chemokine of the CXC subfamily and ligand for the receptor CXCR3. Binding of this protein to CXCR3 results in pleiotropic effects, including stimulation of monocytes, natural killer and T-cell migration, and modulation of adhesion molecule expression. This gene may also be a key regulator of the 'cytokine storm' immune response to SARS-CoSLV2 infection. [provided by RefSeq, Sep 2020]

Function:
Chemotactic for monocytes and T-lymphocytes. Binds to CXCR3.

Subcellular Location:
Secreted.

Post-translational modifications:
CXCL10(1-73) is produced by proteolytic cleavage after secretion from keratinocytes.

Similarity:
Belongs to the intercrine alpha (chemokine CxC) family.

SWISS:
P17515

Gene ID:
15945

Database links:

Entrez Gene: 3627 Human

Entrez Gene: 15945 Mouse

Entrez Gene: 245920 Rat

Omim: 147310 Human

SwissProt: P02778 Human

SwissProt: P17515 Mouse

SwissProt: P48973 Rat

Unigene: 632586 Human

Unigene: 877 Mouse

Unigene: 10584 Rat




取消WB; 2017.4.20; 姬； Picture

Tissue/cell: rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-CXCL10 Polyclonal Antibody, Unconjugated(SL1502R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
C57BL/6 mice skin were fixed in pre-chilled MeOH and incubated at -20℃ for 10 minutes. They were washed in PBS at RT 3 times for 5 minutes each. The sections were blocked for 60 minutes at RT with PBS containing 5% BSA. The block was removed, anti-CXCL10 antibody (SL1502R) diluted 1:50 was added, then incubated overnight at 4℃.Then washed with PBS (0.005% Tween20) for 15 minutes each followed by 2 washes of PBS for 5 minutes each. The secondary antibody, anti-rabbit A488 was diluted 1:500, added to the sections and incubated for 1 hour at RT. Then washed for 10 minutes in PBS 4 times
Blank control: Raw264.7.
Primary Antibody (green line): Rabbit Anti-CXCL10/IP10 antibody (SL1502R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Blank control: Raw264.7.
Primary Antibody (green line): Rabbit Anti-CXCL10/IP10 antibody (SL1502R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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