Binding of anti-Fx1A to Heymann nephritis antigens (HA) on rat glomerular epithelial cells (GECs) in culture leads to capping and disappearance of antigens from the cell surface. This process may contribute to the formation of glomerular subepithelial immune deposits in vivo. The authors differentially extracted GECs to determine whether HA redistribution is mediated by cytoskeletal components. Observations were made by phase-contrast and immunofluorescence microscopy on primary and passaged GECs in monolayer culture and by spectrofluorimetry on GECs in suspension.
Function:
Acts together with cubilin to mediate HDL endocytosis. Receptor-mediated uptake of polybasic drugs such as aprotinin, aminoglycosides and polymyxin B.
Subunit:
Binds plasminogen, extracellular matrix components, plasminogen activator-plasminogen activator inhibitor type I complex, apolipoprotein E-enriched beta-VLDL, lipoprotein lipase, lactoferrin, CLU/clusterin and calcium. Forms a multimeric complex together with a receptor-associated protein (RAP). Binds to ankyrin-repeat family A protein 2 (ANKRA2). Interacts with LRP2BP. Interacts (via NPXY motif) with DAB2; the interaction is not affected by tyrosine phosphorylation of the NPXY motif.
Subcellular Location:
Cell membrane; Single-pass type I membrane protein. Membrane, clathrin-coated pit. Cytoplasm. Note=Expressed in clathrin-coated pits, a soluble form is possibly derived by cleavage at the cell surface.
Tissue Specificity:
Epithelial cells of kidney glomerulus and proximal tubule, lung, epididymis, yolk sac, among others.
Similarity:
Belongs to the LDLR family.
Contains 17 EGF-like domains.
Contains 36 LDL-receptor class A domains.
Contains 37 LDL-receptor class B repeats.
SWISS:
N/A
Gene ID:
361354
Database links:
Entrez Gene: 201456 Human
Entrez Gene: 361354 Rat
Omim: 609093 Human
SwissProt: Q8NCQ5 Human
Unigene: 612811 Human
gp330是肾小管上皮细胞刷状缘上的一种大分子脂蛋白，Fx1A；经生物化学方法纯化后称RTEα5，Fx1A为大分子糖蛋白，近来证实它是—种330Ka的糖蛋白，现称gp330。
主要与肾小球上皮细胞整合素受体作用的抗刷状缘抗体(抗FX1A) 。
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Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Fx1A) Polyclonal Antibody, Unconjugated (SL1338R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat lung); Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Fx1A) Polyclonal Antibody, Unconjugated (SL1338R) at 1:400 overnight at 4°C, followed by a conjugated secondary (Goat Anti-rabbit IgG/Bio) for 20 minutes at 37°C, followed by a conjugated streptavidin (SL0437P-Cy3) at[1:500] for 40 minutes and DAPI staining of the nuclei.
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