This image was generously provided by Xi Qiao from the Second Hospital of Shanxi Medical University. Formaldehyde fixed and paraffin embedded rat kidneys labeled with Rabbit Anti-RAMP1 Polyclonal Antibody (SL1567R) and followed by conjugation to a secondary antibody and counterstained with hematoxylin.
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-RAMP1 Polyclonal Antibody, Unconjugated(SL1567R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining