Home > Product > Antibody > Rabbit Anti-CA I antibody
Carbonic anhydrase I; Carbonic Anhydrase-I; Carbonic Anhydrase 1; Carbonic anhydrase1; Carbonic anhydrase-1; JW0122; CA 1; CA1; CA I; CA-1; CAI; CAN; Car 1; Car1; Carbonate dehydratase I; Carbonic anhydrase 1; Carbonic anhydrase B; Carbonic dehydratase;
Cat:
SL1592R
Species Reactivity:
Mouse,(predicted: Human,Rat,Cow,Rabbit,Sheep,)
Immunogen:
KLH conjugated synthetic peptide derived from human CA I:161-261/261
Format:
Liquid
Storage instructions:
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration:
1mg/ml
Clonality:
Polyclonal
Isotype:
IgG
Applications:
WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500(Paraffin sections need to do antigen repair)not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host:
Rabbit
Product Overview:
Sample: Spleen (Mouse) Lysate at 40 ugPrimary: Anti- CA I (SL1592R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 29 kDObserved band size: 29 kDParaformaldehyde-fixed, paraffin embedded (mouse spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CA I) Polyclonal Antibody, Unconjugated (SL1592R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Unit:
Price: $
Product PDFs
Datasheet:


Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA1 is closely linked to CA2 and CA3 genes on chromosome 8, and it encodes a cytosolic protein which is found at the highest level in erythrocytes. Variants of this gene have been described in some populations. Multiple alternatively spliced variants, encoding the same protein, have been identified. Transcript variants of CA1 utilizing alternative polyA_sites have been described in literature. [provided by RefSeq, Sep 2009]

Function:
Reversible hydration of carbon dioxide.

Subcellular Location:
Cytoplasm.

Similarity:
Belongs to the alpha-carbonic anhydrase family.

SWISS:
P00915

Gene ID:
759

Database links:

Entrez Gene: 759 Human

Entrez Gene: 12346 Mouse

Entrez Gene: 310218 Rat

Omim: 12960 Human

SwissProt: P00915 Human

SwissProt: P13634 Mouse

SwissProt: B0BNN3 Rat

Unigene: 23118 Human

Unigene: 273195 Mouse

Unigene: 6854 Rat



CA1(Carbonic anhydrase1)是红细胞的主要蛋白质成分之一,是一种重要的锌酶,对血液及组织起到维持酸碱平衡的作用,协助体内组织排除二氧化碳,碳酸酐酶I参与呼吸、钙化、酸碱平衡、骨吸收和各种体液形成的生理过程。 Picture

Sample:
Spleen (Mouse) Lysate at 40 ug
Primary: Anti- CA I (SL1592R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 29 kD
Observed band size: 29 kD
Paraformaldehyde-fixed, paraffin embedded (mouse spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CA I) Polyclonal Antibody, Unconjugated (SL1592R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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